| Popis: |
Influence of NRG1, a HER4 ligand, on the HRE4 G1109C mutation. A cellular growth assay using 1% FBS medium and western blotting using serum-free medium were performed. When the transfectant HEK293 cell lines were incubated with serum-free medium, the phosphorylation level of HER4 was also elevated in the HEK293/HER4 G1109C and HEK293/HER4 E317K cell lines, compared with that in the HEK293/HER4 WT cell line. NRG-1 (50 ng/mL) enhanced the cellular growth and phosphorylation level of HER4 in the HEK293/HER4 G1109C and HEK293/HER4 E317K cell lines to extents similar to those in the HEK293/HER4 WT cell line. β-actin was used as an internal control. Column, mean of independent triplicate experiments; error bars, SD; n.s., not significant; *P < 0.05. |
