Comparative Genomic Hybridization Study of de novo Myeloid Neoplasia
| Autor: | Palavarthy H. Rao, Irene Larripa, M. V. Castuma, Susana Acevedo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Monosomy medicine.medical_specialty Adolescent Gene Dosage Trisomy Biology hemic and lymphatic diseases Chromosome regions Complex Karyotype medicine Humans Child In Situ Hybridization Fluorescence Aged Chromosome Aberrations Genetics Cytogenetics Nucleic Acid Hybridization Karyotype Hematology General Medicine Middle Aged Prognosis medicine.disease Chromosome Banding Clone Cells Chromosome 17 (human) Leukemia Myeloid Child Preschool Karyotyping Myelodysplastic Syndromes Acute Disease Cytogenetic Analysis Cancer research Female Comparative genomic hybridization |
| Zdroj: | Acta Haematologica. 104:25-30 |
| ISSN: | 1421-9662 0001-5792 |
| Popis: | Comparative genomic hybridization (CGH) was used to detect chromosomal imbalances in 20 patients with a diagnosis of myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML). The results obtained were compared with G-banding analysis. This last methodology showed 50% of cases with clonal abnormalities whereas CGH detected 70% of cases with copy number changes. Gains were more frequent than losses and constituted 66% of total changes detected. The most common gains included chromosomes 21 and chromosome region 18p for AML and chromosome 17 and region 1p33p35 for MDS. Losses represent 34% of changes and the regions involved were 5q31q32, 7q22, 7p12 and 13q21q22. CGH revealed additional chromosome imbalances in 12 of 20 cases (60%) which were not detected by traditional cytogenetic studies, demonstrating complex karyotype in 50% (6/12). Combination of CGH and G-banding provides an efficient method to identify critical regions present in the malignant clone, which is of great value in the prognosis and outcome of myeloid neoplasias. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|