Physiological and Transcriptional Responses of Saccharomyces cerevisiae to Zinc Limitation in Chemostat Cultures
| Autor: | Graeme M. Walker, Marcel J. T. Reinders, Lucie A. Hazelwood, Michael C. Walsh, Erik de Hulster, Jean-Marc Daran, Pascale Daran-Lapujade, Jack T. Pronk, Theo A. Knijnenburg, Raffaele De Nicola |
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| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic Nitrogen Genes Fungal Saccharomyces cerevisiae Genetics and Molecular Biology Mycology Chemostat Biology Carbohydrate metabolism Response Elements Regulon Applied Microbiology and Biotechnology chemistry.chemical_compound Gene Expression Regulation Fungal Base Sequence Ecology Glycogen Trehalose biology.organism_classification Carbon Yeast Culture Media Oxygen Zinc chemistry Biochemistry Mitochondrial biogenesis Carbohydrate Metabolism Genome Fungal Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 73:7680-7692 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.01445-07 |
| Popis: | Transcriptional responses of the yeast Saccharomyces cerevisiae to Zn availability were investigated at a fixed specific growth rate under limiting and abundant Zn concentrations in chemostat culture. To investigate the context dependency of this transcriptional response and eliminate growth rate-dependent variations in transcription, yeast was grown under several chemostat regimens, resulting in various carbon (glucose), nitrogen (ammonium), zinc, and oxygen supplies. A robust set of genes that responded consistently to Zn limitation was identified, and the set enabled the definition of the Zn-specific Zap1p regulon, comprised of 26 genes and characterized by a broader zinc-responsive element consensus (MHHAACCBYNMRGGT) than so far described. Most surprising was the Zn-dependent regulation of genes involved in storage carbohydrate metabolism. Their concerted down-regulation was physiologically relevant as revealed by a substantial decrease in glycogen and trehalose cellular content under Zn limitation. An unexpectedly large number of genes were synergistically or antagonistically regulated by oxygen and Zn availability. This combinatorial regulation suggested a more prominent involvement of Zn in mitochondrial biogenesis and function than hitherto identified. |
| Databáze: | OpenAIRE |
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