The long non-coding RNA TP73-AS1 modulates HCC cell proliferation through miR-200a-dependent HMGB1/RAGE regulation

Autor: Rong-Rong Zhou, Yongming Fu, Yun Huang, Sha-Ling Li, Yan Huang, Daolin Tang, Rui Kang, Xue Gong Fan
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Cancer Research
Carcinoma
Hepatocellular
Proliferation
Down-Regulation
chemical and pharmacologic phenomena
Real-Time Polymerase Chain Reaction
HMGB1
lcsh:RC254-282
RAGE (receptor)
03 medical and health sciences
miR-200a
0302 clinical medicine
lncRNA
Downregulation and upregulation
Antigens
Neoplasm
Cell Line
Tumor
microRNA
TP73-AS1
Humans
RNA
Antisense
HMGB1 Protein
HCC
Cell Proliferation
Messenger RNA
Gene knockdown
biology
Cell growth
Research
Liver Neoplasms
NF-kappa B
Tumor Protein p73
Hep G2 Cells
Middle Aged
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Antisense RNA
MicroRNAs
030104 developmental biology
Oncology
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Cancer research
biology.protein
Female
RNA
Long Noncoding
Mitogen-Activated Protein Kinases
Zdroj: Journal of Experimental & Clinical Cancer Research, Vol 36, Iss 1, Pp 1-12 (2017)
Journal of Experimental & Clinical Cancer Research : CR
ISSN: 1756-9966
DOI: 10.1186/s13046-017-0519-z
Popis: Background P73 antisense RNA 1 T (non-protein coding), also known as TP73-AS1, is a long non-coding RNA (lncRNA) which is involved in cell proliferation and the development of tumors. However, the exact effects and molecular mechanisms of TP73-AS1 in hepatocellular carcinoma (HCC) progression are still unknown. The present study is aimed to investigate the detailed functions and the mechanism of TP73-AS1 in regulation of HCC cell proliferation. Methods TP73-AS1 expression in HCC tissues and cell lines was determined using real-time PCR assays; the correlation of TP73-AS1 expression with clinicopathological features of HCC was analyzed. The functions of TP73-AS1 in regulation of HCC cell proliferation was evaluated using MTT and BrdU assays. The candidate upstream miRNAs of HMGB1 were screened using miRcode, miRWalk, miRanda and Target scan, verified using real-time PCR assays. The interaction between TP73-AS1 and miR-200a was confirmed using Luciferase report gene assays. The proten levels of HMGB1 signaling-related factors in response to co-processing TP73-AS1 knockdown and miR-200a inhibition were determined using Western blot assays and ELISA. Further, miR-200a, HMGB1 mRNA and RAGE mRNA and their correlations in HCC tissues were determined. Results TP73-AS1 was upregulated in HCC tissues and cell lines. High TP73-AS1 expression was correlated with worse clinicopathological features, poorer prognosis and shorter survival. Knockdown of TP73-AS1 inhibited the HCC proliferation and the expression levels of HMGB1, RAGE and NF-κB in HCC cells. By using online tools, we screened out several candidate upstream miRNAs of HMGB1, among which miR-200a overexpression inhibited HMGB1 mRNA expression the most significantly. By using luciferase assays, we confirmed that miR-200a could directly bind to TP73-AS1 and the 3’UTR of HMGB1; TP73-AS1 competed with HMGB1 for miR-200a binding. MiR-200a inhibition could up-regulate HMGB1, RAGE, NF-κB expression as well as NF-κB regulated cytokines levels, which could be partially restored by si-TP73-AS1. In HCC tissues, miR-200a was down-regulated while HMGB1 and RAGE were up-regulated; TP73-AS1 was inversely correlated with miR-200a, while positively correlated with HMGB1 and RAGE, respectively. Conclusion Our data indicated that TP73-AS1 might be an oncogenic lncRNA that promoted proliferation of HCC and could be regarded as a therapeutic target in human HCC. Electronic supplementary material The online version of this article (doi:10.1186/s13046-017-0519-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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