Major haplotype divergence including multiple germin-like protein genes, at the wheat Sr2 adult plant stem rust resistance locus

Autor: Hana Šimková, Linda Tabe, Rohit Mago, Narayana M. Upadhyaya, Hélène Bergès, Xiuying Kong, James Breen, Marie Kubaláková, Rudi Appels, Jeffrey G. Ellis, Jaroslav Doležel, Sonia Vautrin, Wolfgang Spielmeyer
Přispěvatelé: Agr Flagship, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Centre of the Region Haná for Biotechnological and Agricultural Research [Univ Palacký] (CRH), Faculty of Science [Univ Palacký], Palacky University Olomouc-Palacky University Olomouc-Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), Chinese Academy of Agricultural Sciences (CAAS), Ctr Comparat Genom, Murdoch University, Funding Agency and Grant Number : Czech Science Foundation [P501/12/G090], National Program of Sustainability I [LO1204], Grain Research and Development Corporation [CSP00161]
Jazyk: angličtina
Rok vydání: 2014
Předmět:
inorganic chemicals
haplotype
physical mapping
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Single-nucleotide polymorphism
Locus (genetics)
Plant Science
Plant disease resistance
Stem rust
germin-like proteins (GLPs)
Sr2
comparaison de séquences
Evolution
Molecular
blé
wheat stem rust
Gene family
Coding region
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Gene
Phylogeny
Triticum
Disease Resistance
Glycoproteins
Plant Diseases
Plant Proteins
2. Zero hunger
Genetics
Polymorphism
Genetic
biology
Base Sequence
Basidiomycota
Haplotype
map-based cloning
food and beverages
biology.organism_classification
gène de résistance
Haplotypes
rouille noire
cartographie du génome
gene expression
génétique de la résistance
Puccinia graminis
adult plant resistance (APR)
expression des gènes
Research Article
Zdroj: BMC Plant Biology
BMC Plant Biology, BioMed Central, 2014, 14, article 379 (11 p.). ⟨10.1186/s12870-014-0379-z⟩
BMC Plant Biology (14), article 379 (11 p.). (2014)
ISSN: 1471-2229
DOI: 10.1186/s12870-014-0379-z⟩
Popis: Background The adult plant stem rust resistance gene Sr2 was introgressed into hexaploid wheat cultivar (cv) Marquis from tetraploid emmer wheat cv Yaroslav, to generate stem rust resistant cv Hope in the 1920s. Subsequently, Sr2 has been widely deployed and has provided durable partial resistance to all known races of Puccinia graminis f. sp. tritici. This report describes the physical map of the Sr2-carrying region on the short arm of chromosome 3B of cv Hope and compares the Hope haplotype with non-Sr2 wheat cv Chinese Spring. Results Sr2 was located to a region of 867 kb on chromosome 3B in Hope, which corresponded to a region of 567 kb in Chinese Spring. The Hope Sr2 region carried 34 putative genes but only 17 were annotated in the comparable region of Chinese Spring. The two haplotypes differed by extensive DNA sequence polymorphisms between flanking markers as well as by a major insertion/deletion event including ten Germin-Like Protein (GLP) genes in Hope that were absent in Chinese Spring. Haplotype analysis of a limited number of wheat genotypes of interest showed that all wheat genotypes carrying Sr2 possessed the GLP cluster; while, of those lacking Sr2, some, including Marquis, possessed the cluster, while some lacked it. Thus, this region represents a common presence-absence polymorphism in wheat, with presence of the cluster not correlated with presence of Sr2. Comparison of Hope and Marquis GLP genes on 3BS found no polymorphisms in the coding regions of the ten genes but several SNPs in the shared promoter of one divergently transcribed GLP gene pair and a single SNP downstream of the transcribed region of a second GLP. Conclusion Physical mapping and sequence comparison showed major haplotype divergence at the Sr2 locus between Hope and Chinese Spring. Candidate genes within the Sr2 region of Hope are being evaluated for the ability to confer stem rust resistance. Based on the detailed mapping and sequencing of the locus, we predict that Sr2 does not belong to the NB-LRR gene family and is not related to previously cloned, race non-specific rust resistance genes Lr34 and Yr36. Electronic supplementary material The online version of this article (doi:10.1186/s12870-014-0379-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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