A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia
Autor: | Felisa Alcancia, Barbara K. Goodman, J. Brice Weinberg, Anand S. Lagoo, Nagwa M. Hassanein, Kathryn Perkinson |
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Rok vydání: | 2010 |
Předmět: |
medicine.medical_specialty
Chronic lymphocytic leukemia Concordance DNA Mutational Analysis Immunoglobulin Variable Region chemical and pharmacologic phenomena Biology CD38 Flow cytometry immune system diseases Predictive Value of Tests hemic and lymphatic diseases medicine Biomarkers Tumor Humans Stage (cooking) Neoplasm Staging Chromosome Aberrations Membrane Glycoproteins ZAP-70 Protein-Tyrosine Kinase medicine.diagnostic_test hemic and immune systems Anatomical pathology General Medicine DNA Neoplasm medicine.disease Flow Cytometry Prognosis ADP-ribosyl Cyclase 1 Leukemia Lymphocytic Chronic B-Cell Gene Expression Regulation Immunology Indeterminate Immunoglobulin Heavy Chains Cytometry |
Zdroj: | American journal of clinical pathology. 133(5) |
ISSN: | 1943-7722 |
Popis: | We describe a simple and robust flow cytometry assay for ZAP-70 and CD38 expression. The steps required to validate this assay in a clinical flow cytometry laboratory are described. Two criteria were used to characterize ZAP-70 expression into positive, negative, and indeterminate categories and applied to 111 cases of chronic lymphocytic leukemia (CLL) resulting in 29.7% positive, 56.8% negative, and 13.5% indeterminate cases. A sensitivity-specificity crossover plot between ZAP-70 and CD38 suggested a cutoff of 12.5% for defining CD38 positivity. ZAP-70+ cases were significantly more likely to be at a higher clinical stage and, together with CD38+ cases, were more likely to have unmutated IgV(H). However, for individual patients, the concordance between these markers was not perfect. It may be necessary to evaluate several prognostic markers simultaneously in CLL, and availability of convenient assays for ZAP-70 and CD38 is desirable for optimal clinical decision making. |
Databáze: | OpenAIRE |
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