Somatic motoneurone specification in the hindbrain: the influence of somite-derived signals, retinoic acid and Hoxa3
| Autor: | Sonia Guidato, Fabrice Prin, Sarah Guthrie |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
animal structures
Somatic cell Recombinant Fusion Proteins LIM-Homeodomain Proteins Retinoic acid Rhombomere Hindbrain Nerve Tissue Proteins Tretinoin Chick Embryo Biology Quail Mesoderm chemistry.chemical_compound medicine Animals Humans Brain Tissue Transplantation Cell Lineage Hox gene Molecular Biology In Situ Hybridization Embryonic Induction Homeodomain Proteins Motor Neurons Chimera Neural tube Gene Expression Regulation Developmental Anatomy Zebrafish Proteins Microspheres Cell biology Rhombencephalon Somite medicine.anatomical_structure Electroporation nervous system chemistry Somites embryonic structures Neural plate Developmental Biology Transcription Factors |
| Zdroj: | Development (Cambridge, England). 130(13) |
| ISSN: | 0950-1991 |
| Popis: | We have investigated the mechanisms involved in generating hindbrain motoneurone subtypes, focusing on somatic motoneurones, which are confined to the caudal hindbrain within rhombomeres 5-8. Following heterotopic transplantation of rhombomeres along the rostrocaudal axis at various developmental stages, we have found that the capacity of rhombomeres to generate somatic motoneurones is labile at the neural plate stage but becomes fixed just after neural tube closure, at stage 10-11. Grafting of somites or retinoic acid-loaded beads beneath the rostral hindbrain induced the formation of somatic motoneurones in rhombomere 4 only, and Hox genes normally expressed more caudally (Hoxa3, Hoxd4) were induced in this region. Targeted overexpression of Hoxa3 in the rostral hindbrain led to the generation of ectopic somatic motoneurones in ventral rhombomeres 1-4, and was accompanied by the repression of the dorsoventral patterning gene Irx3. Taken together, these observations suggest that the somites,retinoic acid and Hox genes play a role in patterning somatic motoneurones in vivo. |
| Databáze: | OpenAIRE |
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