Physical effects of tetanus toxin on model membranes containing ganglioside
| Autor: | D. Chapman, Richard J. Cherry, A.W. Clowes |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
food.ingredient
Light Macromolecular Substances medicine.disease_cause Lecithin food Adsorption Cerebrosides Tetanus Toxin Structural Biology Gangliosides medicine Animals Chemical Precipitation Molecular Biology Ganglioside Chromatography Toxin Chemistry Bilayer Electric Conductivity Membranes Artificial Cerebroside Membrane Phosphatidylcholines Cattle Antitoxin Mathematics |
| Zdroj: | Journal of Molecular Biology. 67:49-57 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(72)90385-3 |
| Popis: | Electrical and optical techniques have been used to study the interaction of tetanus toxin with ganglioside in bimolecular lipid membranes. Reflectivity measurements demonstrate that the total thickness of lecithin/ganglioside membranes is 79 ± 2 A. When tetanus toxin is adsorbed onto the surface the thickness is at least 119 A. Lecithin/ganglioside and lecithin/ganglioside/cerebroside membranes have between one and two orders of magnitude lower resistance than lecithin membranes; toxin adsorption produces little further change in the electrical properties. Antitoxin precipitates onto the membrane if added after the toxin has adsorbed on the membrane surface, but not if added before the toxin. From these observations the following conclusions may be drawn: (1) gangliosides in membranes are able to act as receptors for tetanus toxin. (2) The structural integrity of the toxin is maintained following adsorption onto the membrane. (3) The interaction between toxin and membrane is confined to the surface and there is no appreciable modification of the bilayer structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|