DPP-4 inhibition resembles exercise in preventing type 2 diabetes development by inhibiting hepatic protein kinase C expression in a mouse model of hyperinsulinemia
| Autor: | Xiao-fei Han, Chen-xi Li, Xu Liang, Yu-peng Li, Hui Tian, Yu Pei, Jing Xiao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Medicine (General) business.industry Kinase Biochemistry (medical) Type 2 Diabetes Mellitus 030209 endocrinology & metabolism Cell Biology General Medicine Type 2 diabetes medicine.disease Biochemistry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology R5-920 Internal medicine medicine Hyperinsulinemia business HEPATIC PROTEIN Dipeptidyl peptidase-4 |
| Zdroj: | Journal of International Medical Research, Vol 48 (2020) |
| ISSN: | 1473-2300 |
| Popis: | Objective Interventions for hyperinsulinemia (HINS), an early indicator of type 2 diabetes mellitus (T2DM), can significantly reduce the T2DM risk. This study aims to determine how dipeptidyl peptidase-4 (DPP-4) inhibition prevents HINS progression to T2DM through ameliorating hepatic steatosis. Methods KKay mice were used as a HINS model and they underwent exercise or received a DPP-4 inhibitor, MK0626. Hepatic steatosis was examined and liver diacylglycerol levels were determined. Human hepatic cells (LO2) were treated with MK0626 or transfected with DPP-4 siRNA. Protein kinase C ε isoform (PKCε) and DPP-4 expression and insulin receptor substrate 1 (IRS-1) phosphorylation were assessed using immunohistochemistry and western blot. Results KKay mice developed HINS spontaneously at 7 weeks of age. Similar to exercise, MK0626 ameliorated hepatic steatosis and reduced the liver triglyceride and diacylglycerol content. Both exercise and MK0626 suppressed diacylglycerol-induced PKCε expression and restored insulin signaling, which was shown by tyrosine phosphorylation of IRS-1, in the livers of KKay mice. Additionally, silencing DPP-4 or MK0626 treatment decreased PKCε expression in LO2 cells. Conclusions Our data demonstrate that DPP-4 inhibition resembles exercise and effectively delays T2DM onset by suppressing hepatic PKCε expression in the HINS mouse model. |
| Databáze: | OpenAIRE |
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