Effect of the homogenization technique on the enumeration of psychrotrophic bacteria in food absorbent pads
| Autor: | Yves Raymond, Louise Deschênes, Claude P. Champagne, Lang Yao, Marie-Josée Lemay, Ashraf A. Ismail |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Colony-forming unit Bacteriological Techniques Meat Bacteria Absorbent Pads Chemistry Sonication Food Packaging Bacteria Present medicine.disease_cause Microbiology Bacterial Load Cold Temperature Psychrotrophic bacteria Recovery rate Refrigeration medicine Enumeration Food Microbiology Animals Food science Molecular Biology Chickens Homogenization (biology) |
| Zdroj: | Journal of microbiological methods. 187 |
| ISSN: | 1872-8359 |
| Popis: | Four methods were tested for enumerating bacteria present in the absorbent pads (AP) used in packaging chicken and other meats. Viable counts were ascertained at day 0 and day 7 (d0 and d7, respectively). Sampling bacterial cells from AP were carried out using a countertop blender, Stomacher, sonication, and blender in combination to sonication. The release of bacterial cells by breaking down the AP with the blender resulted in the highest CFU counts. At d0, a bacterial recovery rate of 94% was obtained with the blender, while the recovery rates using Stomacher or sonication alone were 58% and 73%, respectively. At d7, the Stomacher treatment also gave the lowest colony forming unit (CFU) values in the AP incubated at 7 °C. Sonication of the AP prior to homogenization with the blender did not increase CFU counts. Results suggested that breaking down the AP with a blender gives higher CFU levels than the Stomacher, which is the most commonly used technique for this purpose. |
| Databáze: | OpenAIRE |
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