Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells
| Autor: | Ilan Calderon, David K. Gardner, Michelle Lane, John Leeton |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
media_common.quotation_subject Metabolite Luteal phase Biology chemistry.chemical_compound Internal medicine Pyruvic Acid Follicular phase medicine Humans Embryo Implantation Lactic Acid Pyruvates Ovulation Cells Cultured Fallopian Tubes media_common Uterus Obstetrics and Gynecology Metabolism Embryo Mammalian Body Fluids Lactic acid Glucose Endocrinology Reproductive Medicine chemistry Lactates Oocytes Oviduct Female Pyruvic acid |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0015-0282 |
| Popis: | Objective To determine the levels of metabolites surrounding the human oocyte and embryo in vivo. Design Oviduct and uterine fluids were collected throughout the menstrual cycle. Cumulus cells were collected at oocyte retrieval and their production of metabolites was assessed. Samples were analyzed for pyruvate, lactate, and glucose by microfluorimetry. Patients Luminal fluids were collected from naturally cycling patients at the time of routine clinical investigation. Patient consent and hospital ethics approval were obtained for this study. Results Pyruvate in the oviduct did not vary with the day of cycle, the mean value was 0.24 mM. Lactate and glucose concentrations varied with the day of cycle; lactate increasing from 4.87 mM in the follicular phase to 10.50 mM at the time of ovulation, whereas glucose decreased from 3.11 mM in the follicular phase to 0.50 mM midcycle and subsequently increased to 2.32 mM in the luteal phase. The concentrations of pyruvate, lactate, and glucose in uterine fluid remained constant throughout the cycle (0.10, 5.87, and 3.15 mM, respectively). All metabolite concentrations in uterine fluid were significantly different from those in the oviduct midcycle. Cumulus cells readily consumed glucose in vitro, with lactate being the major metabolite produced. Conclusion These data indicate that lactate and glucose concentrations in the oviduct change with day of cycle and that the human embryo is exposed to different metabolite concentrations as it passes along the tract. Furthermore, cumulus cells readily consume glucose, producing lactate. Therefore, the early human embryo is exposed to low glucose and high lactate levels in vivo. |
| Databáze: | OpenAIRE |
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