Circular RNA circ_0000592 elevates ANXA4 expression via sponging miR-1179 to facilitate tumor progression in gastric cancer
| Autor: | Weiying Diao, Zhiwu Ji, Jincai Shang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Untranslated region
Male Cancer Research Mice Nude Mice Gentamicin protection assay Cell Movement Stomach Neoplasms Cell Line Tumor Gene silencing Animals Humans Pharmacology (medical) Lactic Acid Annexin A4 Cell Proliferation Pharmacology Gene knockdown Mice Inbred BALB C Migration Assay Cell growth Chemistry RNA RNA Circular Xenograft Model Antitumor Assays Tumor Burden MicroRNAs Oncology Tumor progression Cancer research Glycolysis |
| Zdroj: | Anti-cancer drugs. 33(1) |
| ISSN: | 1473-5741 |
| Popis: | Increasing evidence indicated that dysregulated circular RNAs were implicated in the progression of multiple malignancies. However, the function of circ_0000592 in gastric cancer (GC) progression and its associated mechanism remain poorly understood. Quantitative real-time PCR and Western blot assay were performed to detect RNA and protein expression. Cell proliferation, migration and invasion were analyzed by 5-Ethynyl-2'-deoxyuridine staining assay, Transwell migration assay and Transwell invasion assay, respectively. The glucose/lactate assay kit was used to assess the rates of glucose consumption and lactate production. The interaction between microRNA-1179 (miR-1179) and circ_0000592 or Annexin A4 (ANXA4) was confirmed by dual-luciferase reporter assay and RNA pull-down assay. Xenograft tumor model was established to investigate the effect of circ_0000592 on tumor growth in vivo. Circ_0000592 expression was elevated in GC tissues and cells. Circ_0000592 knockdown hampered cell proliferation, migration, invasion and glycolysis of GC cells. MiR-1179 was a direct target of circ_0000592, and circ_0000592 silencing-mediated effects in GC cells were partly reversed by the knockdown of miR-1179. MiR-1179 interacted with the 3' untranslated region (3'UTR) of ANXA4. Circ_0000592 silencing reduced ANXA4 expression partly by upregulating miR-1179 in GC cells. ANXA4 overexpression partly overturned circ_0000592 knockdown-induced effects in GC cells. Circ_0000592 depletion markedly suppressed xenograft tumor growth in vivo. Circ_0000592 contributed to GC progression through regulating miR-1179/ANXA4 axis, which provided novel potential biomarkers and therapeutic targets for GC treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|