Crumbs is required to achieve proper organ size control duringDrosophilahead development
| Autor: | Emily C. N. Richardson, Franck Pichaud |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Mutant
Morphogenesis Genes Insect Biology Eye Endocytosis Animals Genetically Modified Adherens junction Animals Drosophila Proteins Wings Animal Molecular Biology DNA Primers Epithelial polarity Genetics Base Sequence Receptors Notch Membrane Proteins Organ Size Cell biology Imaginal disc Phenotype Mutation Microscopy Electron Scanning Drosophila Photoreceptor Cells Invertebrate Head Developmental biology Drosophila Protein Research Article Signal Transduction Developmental Biology |
| Zdroj: | Development. 137:641-650 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Crumbs (Crb) is a conserved apical polarity determinant required for zonula adherens specification and remodelling during Drosophila development. Interestingly, crb function in maintaining apicobasal polarity appears largely dispensable in primary epithelia such as the imaginal discs. Here, we show that crb function is not required for maintaining epithelial integrity during the morphogenesis of the Drosophila head and eye. However, although crb mutant heads are properly developed, they are also significantly larger than their wild-type counterparts. We demonstrate that in the eye, this is caused by an increase in cell proliferation that can be attributed to an increase in ligand-dependent Notch (N) signalling. Moreover, we show that in crb mutant cells, ectopic N activity correlates with an increase in N and Delta endocytosis. These data indicate a role for Crb in modulating endocytosis at the apical epithelial plasma membrane, which we demonstrate is independent of Crb function in apicobasal polarity. Overall, our work reveals a novel function for Crb in limiting ligand-dependent transactivation of the N receptor at the epithelial cell membrane. |
| Databáze: | OpenAIRE |
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