Human immune cells' behavior and survival under bioenergetically restricted conditions in an in vitro fracture hematoma model
| Autor: | Tabea Schütze, Andrea Ode, Saskia Schellmann, Gerd-Rüdiger Burmester, René Dziurla, Timo Gaber, Paula Hoff, Katharina Schmidt-Bleek, Patrick Maschmeyer, Tobias Raue, Georg N. Duda, Frank Buttgereit, Ferenz Leonard Lohanatha, Carsten Perka, Eric Röhner |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Myeloid Cell Survival Arthroplasty Replacement Hip Immunology Bone healing Biology Fractures Bone Interferon-gamma Immune system medicine Immunology and Allergy Humans Femur Cells Cultured Chemokine CCL2 Aged Hematoma Wound Healing Innate immune system Interleukin-6 Interleukin-8 Middle Aged Acquired immune system Immunopharmacology Transplantation Infectious Diseases Neuroimmunology medicine.anatomical_structure Female Energy Metabolism Research Article |
| Zdroj: | Cellularmolecular immunology. 10(2) |
| ISSN: | 2042-0226 |
| Popis: | The initial inflammatory phase of bone fracture healing represents a critical step for the outcome of the healing process. However, both the mechanisms initiating this inflammatory phase and the function of immune cells present at the fracture site are poorly understood. In order to study the early events within a fracture hematoma, we established an in vitro fracture hematoma model: we cultured hematomas forming during an osteotomy (artificial bone fracture) of the femur during total hip arthroplasty (THA) in vitro under bioenergetically controlled conditions. This model allowed us to monitor immune cell populations, cell survival and cytokine expression during the early phase following a fracture. Moreover, this model enabled us to change the bioenergetical conditions in order to mimic the in vivo situation, which is assumed to be characterized by hypoxia and restricted amounts of nutrients. Using this model, we found that immune cells adapt to hypoxia via the expression of angiogenic factors, chemoattractants and pro-inflammatory molecules. In addition, combined restriction of oxygen and nutrient supply enhanced the selective survival of lymphocytes in comparison with that of myeloid derived cells (i.e., neutrophils). Of note, non-restricted bioenergetical conditions did not show any similar effects regarding cytokine expression and/or different survival rates of immune cell subsets. In conclusion, we found that the bioenergetical conditions are among the crucial factors inducing the initial inflammatory phase of fracture healing and are thus a critical step for influencing survival and function of immune cells in the early fracture hematoma. |
| Databáze: | OpenAIRE |
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