sodVIC is an alpha-COP-related gene which is essential for establishing and maintaining polarized growth in Aspergillus nidulans

Autor: John H. Doonan, Susan J. Assinder, Susan L. Whittaker, P. Lunness, K.J. Milward
Rok vydání: 1999
Předmět:
Zdroj: Fungal genetics and biology : FGB. 26(3)
ISSN: 1087-1845
Popis: Strains of Aspergillus nidulans carrying the conditional-lethal mutation sod VI C1 (stabilization of disomy) are defective in nuclear division and hyphal extension. The mutation affects both the establishment and maintenance of polar growth, since mutant spores do not germinate at restrictive temperature and preexisting hyphae stop growing upon upshift. The defect is reversible within the first 3–4 h at restrictive temperature but longer periods of incubation are lethal due to cell lysis and morphological abnormalities. There is no evidence for a specific cell cycle lesion, suggesting the existence of a feedback mechanism whereby hyphal extension is coordinated with nuclear partitioning. The sod VI C gene has been cloned from a chromosome VI-specific cosmid library and its product exhibits strong homology to the α-COP subunit of the coatomer complex involved in the secretory pathway in yeast and higher organisms. Molecular disruption of the gene is lethal, indicating that Sod VI C is essential for growth in A. nidulans.
Databáze: OpenAIRE