Deficiency of phytochrome B alleviates chilling‐induced photoinhibition in rice
| Autor: | Guoliang Han, Na Sui, Xian-Zhi Xie, Bao-Shan Wang, Jian-Chao Yang, Meng Li |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Chlorophyll
Chloroplasts Photoinhibition Light Photosystem II Plant Science Xanthophylls Biology Photosynthesis Models Biological Electron Transport Microscopy Electron Transmission Phytochrome B Genetics RNA Messenger Ecology Evolution Behavior and Systematics Unsaturated fatty acid chemistry.chemical_classification P700 Photosystem I Protein Complex Phytochrome Fatty Acids Photosystem II Protein Complex food and beverages Oryza Plants Genetically Modified Cold Temperature Plant Leaves Chloroplast Phenotype chemistry Biochemistry RNA Plant Xanthophyll Mutation |
| Zdroj: | American Journal of Botany. 100:1860-1870 |
| ISSN: | 1537-2197 0002-9122 |
| DOI: | 10.3732/ajb.1200574 |
| Popis: | UNLABELLED PREMISE OF THE STUDY Food crops of tropical origins, such as rice, are often cultivated in areas with suboptimal temperature regimes. The rice phytochrome B-deficient mutant (phyB) is tolerant of chilling temperatures compared with the wild type (WT) under low irradiance, and unsaturated fatty acids (USFAs) of membrane lipids have been shown to play an important role in chilling resistance. However, the relationship between phytochrome B and membrane lipids has not been empirically investigated. • METHODS We assessed various photosynthesis indexes in phyB and WT rice: chlorophyll content, maximal photochemical efficiency (Fv/Fm) of photosystem II (PSII), the quantum yield of PSII electron transport (ΦPSII), the percentage of oxidizable P700 (P700), nonphotochemical quenching (NPQ), and the de-epoxidized ratio of xanthophyll cycle (A+Z)/(V+A+Z). We also analyzed the ultrastructure and fatty acid desaturases (FADs) and glycerol-3-phosphate acyltransferase (GPAT) genes of the chloroplasts using transmission electron microscopy and real-time PCR. • RESULTS After a chilling treatment of 24 h, chloroplast damage and USFA content reduction were more severe in the WT than in the phyB mutant. Genes involved in the synthesis of USFAs in membranes such as FADs and GPAT were more stable in phyB than in WT. Chlorophyll content, Fv/Fm, ΦPSII, and P700 decreased more slowly under chilling stress and recovered more rapidly under optimal conditions in phyB than in WT. The (A+Z)/(V+A+Z) and NPQ increased more rapidly in phyB than in the WT after 24 h of chilling treatment. • CONCLUSIONS Phytochrome B deficiency in rice with more stabilized chloroplast structure and higher USFA content in membrane lipids could alleviate chilling-induced photoinhibition. |
| Databáze: | OpenAIRE |
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