Ultrasound combined with manganese-oxide nanoparticles loaded on activated carbon for extraction and pre-concentration of thymol and carvacrol in methanolic extracts of Thymus daenensis, Salvia officinalis, Stachys pilifera, Satureja khuzistanica, and mentha, and water samples
| Autor: | Arash Asfaram, Hossein Sadeghi, Alireza Goudarzi, Esmaeel Panahi Kokhdan, Zeinab Salehpour |
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| Rok vydání: | 2019 |
| Předmět: |
Central composite design
02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Matrix (chemical analysis) Thymus Plant chemistry.chemical_compound Sonication food Limit of Detection Electrochemistry Environmental Chemistry Carvacrol Salvia officinalis Thymol Spectroscopy Detection limit Chromatography Chemistry Plant Extracts Methanol Satureja 010401 analytical chemistry Extraction (chemistry) Solid Phase Extraction Oxides Repeatability 021001 nanoscience & nanotechnology food.food Carbon 0104 chemical sciences Manganese Compounds Monoterpenes Cymenes Nanoparticles Stachys 0210 nano-technology Water Pollutants Chemical Mentha |
| Zdroj: | The Analyst. 144(6) |
| ISSN: | 1364-5528 |
| Popis: | A dispersive micro solid-phase extraction (DMSPE) technique was developed using manganese-oxide nanoparticles loaded on activated carbon (Mn3O4-NPs-AC) as an effective sorbent combined with ultrasound for the extraction and determination of a trace amount of thymol and carvacrol in methanolic extracts of Thymus daenensis, Salvia officinalis, Stachys pilifera, Satureja khuzistanica and mentha, and water samples. Thymol and carvacrol phenolic compounds were extracted from real samples using acetonitrile (ACN) as the desorption solvent. Using central composite design (CCD), the effects of pH, ionic strength (NaCl), nano-sorbent mass, contact time, and desorption volume were investigated. Additionally, based on five-level variables, response surface methodology was used to determine the individual and interactive effects between factors on the process. The optimized extraction conditions included 12 mg of Mn3O4-NPs-AC as the sorbent, 300 μL of ACN as the desorption solvent, pH 3.0, 0.5 w/v% of NaCl, and 4.5 min sonication time. Under the optimized conditions, for all the samples, the limits of detection were 0.054-0.104 ng mL-1 and the limits of quantification were 0.178-0.345 ng mL-1. The correlation coefficients of the calibration curves were >0.985, i.e. in the range of 0.4-6000 ng mL-1. To validate the effects of the matrix, the recovery, reproducibility, repeatability, and overall uncertainty were calculated for the five methanolic extracts, at 50, 100, and 500 ng mL-1. The recovery ranged between 94.5% and 109.0% with a relative standard deviation of |
| Databáze: | OpenAIRE |
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