Characterisation of microbiota in saliva, bronchoalveolar lavage fluid, non-malignant, peritumoural and tumour tissue in non-small cell lung cancer patients: a cross-sectional clinical trial

Autor: Marie-Paule Vasson, Edith Filaire, Eve Delmas, Ioana Molnar, Marc Filaire, Rea Bingula, Annick Bernalier-Donadille, Jean-Yves Berthon
Přispěvatelé: Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biopole Clermont-Limagne, ARVALIS - Institut du végétal [Paris], Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Laboratoire d'analyse des interfaces et de nanophysique (LAIN), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Microbiologie Environnement Digestif Santé (MEDIS), DRV/VALO 2017-033Greentech SA (FR)DRV/VALO 2017-033European Regional Development FundDRV/VALO 2017-033Region Auvergne-Rhone-Alpes, ROSSI, Sabine
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Saliva
Pathology
Lung Neoplasms
Lobe location
Lower lobe tumour
0302 clinical medicine
Non-small cell lung cancer
Carcinoma
Non-Small-Cell Lung
Prospective Studies
Peritumoural lung tissue
biology
medicine.diagnostic_test
Microbiota
respiratory system
Middle Aged
3. Good health
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
Lung cancer
Proteobacteria
Bronchoalveolar Lavage Fluid
Bronchoalveolar lavage
medicine.medical_specialty
Firmicutes
Actinobacteria
03 medical and health sciences
medicine
Humans
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Aged
lcsh:RC705-779
Lung
Research
Non-malignant lung tissue
lcsh:Diseases of the respiratory system
biology.organism_classification
medicine.disease
respiratory tract diseases
stomatognathic diseases
Cross-Sectional Studies
030104 developmental biology
Lung microbiota
Flavobacteriia
Zdroj: Respiratory Research
Respiratory Research, 2020, 21 (1), ⟨10.1186/s12931-020-01392-2⟩
Respiratory Research, BioMed Central, 2020, 21 (1), ⟨10.1186/s12931-020-01392-2⟩
Respiratory Research, Vol 21, Iss 1, Pp 1-20 (2020)
ISSN: 1465-993X
1465-9921
Popis: Background While well-characterised on its molecular base, non-small cell lung cancer (NSCLC) and its interaction with local microbiota remains scarcely explored. Moreover, current studies vary in source of lung microbiota, from bronchoalveolar lavage fluid (BAL) to tissue, introducing potentially differing results. Therefore, the objective of this study was to provide detailed characterisation of the oral and multi-source lung microbiota of direct interest in lung cancer research. Since lung tumours in lower lobes (LL) have been associated with decreased survival, characteristics of the microbiota in upper (UL) and lower tumour lobes have also been examined. Methods Using 16S rRNA gene sequencing technology, we analysed microbiota in saliva, BAL (obtained directly on excised lobe), non-malignant, peritumoural and tumour tissue from 18 NSCLC patients eligible for surgical treatment. Detailed taxonomy, diversity and core members were provided for each microbiota, with analysis of differential abundance on all taxonomical levels (zero-inflated binomial general linear model with Benjamini-Hochberg correction), between samples and lobe locations. Results Diversity and differential abundance analysis showed clear separation of oral and lung microbiota, but more importantly, of BAL and lung tissue microbiota. Phylum Proteobacteria dominated tissue samples, while Firmicutes was more abundant in BAL and saliva (with class Clostridia and Bacilli, respectively). However, all samples showed increased abundance of phylum Firmicutes in LL, with decrease in Proteobacteria. Also, clades Actinobacteria and Flavobacteriia showed inverse abundance between BAL and extratumoural tissues depending on the lobe location. While tumour microbiota seemed the least affected by location, peritumoural tissue showed the highest susceptibility with markedly increased similarity to BAL microbiota in UL. Differences between the three lung tissues were however very limited. Conclusions Our results confirm that BAL harbours unique lung microbiota and emphasise the importance of the sample choice for lung microbiota analysis. Further, limited differences between the tissues indicate that different local tumour-related factors, such as tumour type, stage or associated immunity, might be the ones responsible for microbiota-shaping effect. Finally, the “shift” towards Firmicutes in LL might be a sign of increased pathogenicity, as suggested in similar malignancies, and connected to worse prognosis of the LL tumours. Trial registration ClinicalTrials.gov ID: NCT03068663. Registered February 27, 2017.
Databáze: OpenAIRE
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