Roles of Glucose in Photoreceptor Survival*
| Autor: | Drew Couron, Jonathan D. Linton, Iok Teng Kuok, Edward D Parker, Andrei O. Chertov, James B. Hurley, Martin Sadilek, Lars C. Holzhausen, Ian R. Sweet |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Programmed cell death
genetic structures Cell Survival Carbohydrate metabolism Mitochondrion Biology Biochemistry Gas Chromatography-Mass Spectrometry Retina Acetylglucosamine chemistry.chemical_compound Mice Adenosine Triphosphate Oxygen Consumption Neurobiology medicine Autophagy Animals Lactic Acid Molecular Biology Neurons Cell Death Neurodegeneration Neurodegenerative Diseases Cell Biology medicine.disease NAD eye diseases Cell biology Mitochondria Citric acid cycle Mice Inbred C57BL Glucose chemistry nervous system NAD+ kinase sense organs Adenosine triphosphate Photoreceptor Cells Vertebrate |
| Popis: | Vertebrate photoreceptor neurons have a high demand for metabolic energy, and their viability is very sensitive to genetic and environmental perturbations. We investigated the relationship between energy metabolism and cell death by evaluating the metabolic effects of glucose deprivation on mouse photoreceptors. Oxygen consumption, lactate production, ATP, NADH/NAD(+), TCA cycle intermediates, morphological changes, autophagy, and viability were evaluated. We compared retinas incubated with glucose to retinas deprived of glucose or retinas treated with a mixture of mitochondrion-specific fuels. Rapid and slow phases of cell death were identified. The rapid phase is linked to reduced mitochondrial activity, and the slower phase reflects a need for substrates for cell maintenance and repair. |
| Databáze: | OpenAIRE |
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