Simultaneous HPLC gradient analysis of 15 benzodiazepines and selected metabolites in postmortem blood
| Autor: | Allison W. Peace, Soumela Horomidis, Marie L. Syrjanen, Olaf H. Drummer, Kerryn Crump, Iain M. McIntyre |
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| Rok vydání: | 1993 |
| Předmět: |
Chemical Health and Safety
Chromatography Gradient analysis Health Toxicology and Mutagenesis Extraction (chemistry) Reproducibility of Results Ether Postmortem blood Forensic Medicine Toxicology High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry Solvent chemistry.chemical_compound Benzodiazepines chemistry Potassium phosphate Environmental Chemistry Humans Acetonitrile Blood Chemical Analysis Chromatography High Pressure Liquid |
| Zdroj: | Journal of analytical toxicology. 17(4) |
| ISSN: | 0146-4760 |
| Popis: | The simultaneous identification and quantitation of 15 benzodiazepines and selected metabolites in postmortem blood, serum, or liver homogenate is described. The assay involves extraction with diethylether, followed by an acid clean-up step of the ether. Chromatographic separation was achieved on a Nova-Pak phenyl 18 column using ultraviolet detection at 240 nm. A gradient HPLC system was developed to improve separation of nitro-reduction metabolites from the solvent front and endogenous peaks. The mobile phases consisted of a gradient from 15 to 28% acetonitrile in 40 mM potassium phosphate buffer. Within-run and day-to-day precision were generally 10-15%. The method described is sensitive and reproducible for the analysis of benzodiazepine concentrations in postmortem tissues. |
| Databáze: | OpenAIRE |
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