A P19 and P19CL6 Cell-Based Complementary Approach to Determine Paracrine Effects in Cardiac Tissue Engineering
| Autor: | Eva-Maria Materne, Jochen Ringe, Michael Sittinger, Tilo Dehne, Sophie Van Linthout, Marion Haag, Jan Philipp Krüger, Carsten Tschöpe, Xavier Adam, Mareike Carola Reimann |
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| Rok vydání: | 2014 |
| Předmět: |
Histology
Cell Connexin Biology Mice Paracrine signalling Tissue engineering Carcinoma Embryonal Cell Line Tumor medicine Animals Humans Myocytes Cardiac Tissue Engineering Cell growth Myocardium Cell Differentiation Heart Embryonic stem cell Coculture Techniques In vitro Cell biology medicine.anatomical_structure P19 cell Culture Media Conditioned Immunology Anatomy |
| Zdroj: | Cells Tissues Organs. 199:24-36 |
| ISSN: | 1422-6421 1422-6405 |
| DOI: | 10.1159/000362540 |
| Popis: | The negligible self-repair potential of the myocardium has led to cell-based tissue engineering approaches to restore heart function. There is more and more consensus that, in addition to cell development, paracrine effects in particular play a pivotal role in the repair of heart tissue. Here, we present two complementary murine P19 and P19CL6 embryonic carcinoma cell-based in vitro test approaches to study the potential of repair cells and the factors secreted by these cells to induce cardiomyogenesis. P19 cells were 3-dimensionally cultured in hanging drops and P19CL6 cells in a monolayer. Both systems, capable of inducible differentiation towards the cardiomyogenic lineage shown by the appearance of beating cells, the expression of connexin 43 and cardiac troponins T and I, were used to test the cardiomyogenesis-inducing potential of human cardiac-derived adherent proliferating (CardAP) cells, which are candidates for heart repair. CardAP cells in coculture as well as CardAP cell-conditioned medium initiated beating in P19 cells, depending on the cell composition and concentration of the medium. CardAP cell-dependent beating was not observed in P19CL6 cultures, but connexin 43 and cardiac troponin formation as well as expression of GATA-binding protein 4 indicated the dose-dependent stimulatory cardiomyogenic effect of human CardAP cells. In summary, in different ways, P19 and P19CL6 cells have shown their capability to detect paracrine effects of human CardAP cells. In a complementary approach, they could be beneficial for determining the stimulatory cardiomyogenic potential of candidate cardiac-repair cells in vitro. |
| Databáze: | OpenAIRE |
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