HIV-1 tracing method of systemic viremia in vivo using an artificially mutated virus pool
| Autor: | Andrew Soper, Yoshio Koyanagi, Kei Sato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
CD4-Positive T-Lymphocytes
Immunology Mutagenesis (molecular biology technique) Viremia Spleen HIV Infections Biology Virus Replication Microbiology Virus 03 medical and health sciences Jurkat Cells Mice In vivo Virology medicine Animals Humans nef Gene Products Human Immunodeficiency Virus 030304 developmental biology 0303 health sciences 030306 microbiology virus diseases medicine.disease Haematopoiesis medicine.anatomical_structure Lymphatic system HIV-1 RNA Viral Bone marrow |
| Zdroj: | Microbiology and immunologyREFERENCES. 65(1) |
| ISSN: | 1348-0421 |
| Popis: | The appearance of human immunodeficiency virus type 1 (HIV-1) plasma viremia is associated with progression to symptomatic disease and CD4+ T cell depletion. To locate the source of systemic viremia, this study employed a novel method to trace HIV-1 infection in vivo. We created JRCSFξnef, a pool of infectious HIV-1 (strain JR-CSF) with highly mutated nef gene regions by random mutagenesis PCR and infected this mutated virus pool into both Jurkat-CCR5 cells and hematopoietic stem cell-transplanted humanized mice. Infection resulted in systemic plasma viremia in humanized mice and viral RNA sequencing helped us to identify multiple lymphoid organs such as spleen, lymph nodes, and bone marrow but not peripheral blood cells as the source of systemic viremia. Our data suggest that this method could be useful for the tracing of viral trafficking in vivo. |
| Databáze: | OpenAIRE |
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