Differential Effects of Progenitor Cell Populations on Left Ventricular Remodeling and Myocardial Neovascularization After Myocardial Infarction
| Autor: | Marinee Chuah, Stefan Janssens, Christophe Dubois, Lertlak Chaothawee, Jan Bogaert, Desire Collen, Piet Claus, Hilde Gillijns, Witold Streb, Peter Pokreisz, Sara Vandenwijngaert, Olivier Gheysens, Ann Belmans, Frans Van de Werf, Zeger Debyser, Glenn Marsboom, Hélène Dépelteau, Frederik Maes, Erik Verbeken, Thierry VandenDriessche, Xiaoshun Liu, Marijke Pellens |
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| Přispěvatelé: | Division of Gene Therapy & Regenerative Medicine, Cell Biology and Histology |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Magnetic Resonance Imaging Cine Neovascularization Physiologic Myocardial Reperfusion 030204 cardiovascular system & hematology Mesenchymal Stem Cell Transplantation Endothelial progenitor cell Neovascularization 03 medical and health sciences 0302 clinical medicine Internal medicine Paracrine Communication medicine Animals magnetic resonance imaging Myocardial infarction Progenitor cell Ventricular remodeling Cells Cultured endothelial progenitor cells 030304 developmental biology left ventricular remodeling Tube formation 0303 health sciences Ejection fraction Ventricular Remodeling Reverse Transcriptase Polymerase Chain Reaction business.industry Mesenchymal Stem Cells myocardial neovascularization medicine.disease Immunohistochemistry Matrix Metalloproteinases myocardial infarction Lac Operon Heart failure Cardiology cell therapy medicine.symptom business Cardiology and Cardiovascular Medicine Stem Cell Transplantation |
| Zdroj: | Journal of the American College of Cardiology Journal of the American College of Cardiology; Vol 55 |
| ISSN: | 0735-1097 |
| DOI: | 10.1016/j.jacc.2009.10.081 |
| Popis: | OBJECTIVES: We compared biological repair after acute myocardial infarction (AMI) with selected porcine progenitor cell populations. BACKGROUND: Cell types and mechanisms responsible for myocardial repair after AMI remain uncertain. METHODS: In a blinded, randomized study, we infused autologous late-outgrowth endothelial progenitor cells (EPC) (n = 10, 34 +/- 22 x 10(6) CD29-31-positive, capable of tube formation), allogeneic green fluorescent peptide-labeled mesenchymal stem cells (MSC) (n = 11, 10 +/- 2 x 10(6) CD29-44-90-positive, capable of adipogenic and osteogenic differentiation), or vehicle (CON) (n = 12) in the circumflex artery 1 week after AMI. Systolic function (ejection fraction), left ventricular (LV) end-diastolic and end-systolic volumes, and infarct size were assessed with magnetic resonance imaging at 1 week and 7 weeks. Cell engraftment and vascular density were evaluated on postmortem sections. RESULTS: Recovery of LV ejection fraction from 1 to 7 weeks was similar between groups, but LV remodeling markedly differed with a greater increase of LV end-systolic volume in MSC and CON (+11 +/- 12 ml/m(2) and +7 +/- 8 ml/m(2) vs. -3 +/- 11 ml/m(2) in EPC, respectively, p = 0.04), and a similar trend was noted for LV end-diastolic volume (p = 0.09). After EPC, infarct size decreased more in segments with >50% infarct transmurality (p = 0.02 vs. MSC and CON) and was associated with a greater vascular density (p = 0.01). Late outgrowth EPCs secrete higher levels of the pro-angiogenic placental growth factor (733 [277 to 1,214] pg/10(6) vs. 59 [34 to 88] pg/10(6) cells in MSC, p = 0.03) and incorporate in neovessels in vivo. CONCLUSIONS: Infusion of late-outgrowth EPCs after AMI improves myocardial infarction remodeling via enhanced neovascularization but does not mediate cardiomyogenesis. Endothelial progenitor cell transfer might hold promise for heart failure prevention via pro-angiogenic or paracrine matrix-modulating effects. |
| Databáze: | OpenAIRE |
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