Metabolite profiling of Epinephelus fuscoguttatus infected with vibriosis reveals Omega 9 as potential metabolite biomarker
| Autor: | Yosmetha Mayalvanan, A’wani Aziz Nurdalila, Syarul Nataqain Baharum |
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| Rok vydání: | 2018 |
| Předmět: |
Physiology
Metabolite Vibrio vulnificus Aquatic Science Biochemistry 03 medical and health sciences chemistry.chemical_compound Fish Diseases Tiger grouper Palmitoleic acid Animals Food science 030304 developmental biology chemistry.chemical_classification 0303 health sciences Epinephelus fuscoguttatus biology Fatty Acids Fatty acid 04 agricultural and veterinary sciences General Medicine biology.organism_classification Perciformes Oleic acid chemistry Vibrio Infections 040102 fisheries 0401 agriculture forestry and fisheries Gas chromatography–mass spectrometry Food Deprivation Biomarkers |
| Zdroj: | Fish physiology and biochemistry. 45(3) |
| ISSN: | 1573-5168 |
| Popis: | In this study, we report the starvation effect and vibriosis infection on a tropical fish, the tiger grouper (Epinephelus fuscoguttatus). The tiger groupers were infected with Vibrio vulnificus for 21 days. Gas chromatography-mass spectrometry combined with multivariate analysis was used to assess the variation in metabolite profiles of E. fuscoguttatus. Metabolite productions in infected fishes were significantly influenced by fatty acid production. The Omega 9 (ω-9) was abundant under the challenged conditions compared to Omega 3 (ω-3) and Omega 6 (ω-6). A total of six fatty acids from the ω-9 group were detected in high concentration in the infected fishes compared to the control groupers. These metabolites are Oleic acid, Palmitoleic acid, 6,9-Octadecenoic acid, 8,11-Eicosadienoic acid, cis-Erucic acid and 5,8,11-Eicosatrienoic acid. The production of ω-9 differed significantly (p ≤ 0.001) in the challenged samples. The detected ω-9 compounds were quantified based on three different extraction techniques with Supelco 37-component FAME mix (Supelco, USA). The highest concentration of ω-9 groups compared to the other fatty acids detected is 1320.79 mg/4 g and the lowest is 939 mg/4 g in challenged-starved; meanwhile, in challenged-fed, the highest concentration detected is 1220.87 mg/4 g and the lowest is 917.25 mg/4 g. These changes demonstrate that ω-9 can be used as a biomarker of infection in fish. |
| Databáze: | OpenAIRE |
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