Phospholipid bilayers supported on thiolate-covered nanostructured gold: in situ Raman spectroscopy and electrochemistry of redox species
| Autor: | Alejandro Fainstein, Roberto Carlos Salvarezza, Nicolás G. Tognalli, María Elena Vela, Tânia Beatriz Creczynski-Pasa, M. Antonieta Daza Millone |
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| Rok vydání: | 2009 |
| Předmět: |
Vesicle fusion
Lipid Bilayers Analytical chemistry Phospholipid Photochemistry Spectrum Analysis Raman symbols.namesake chemistry.chemical_compound Electrochemistry Sulfhydryl Compounds Physical and Theoretical Chemistry Lipid bilayer chemistry.chemical_classification Chemistry Bilayer Biomolecule Vesicle Atomic and Molecular Physics and Optics Nanostructures Methylene Blue Dithiothreitol Membrane symbols Flavin-Adenine Dinucleotide Gold Raman spectroscopy Dimyristoylphosphatidylcholine Oxidation-Reduction |
| Zdroj: | Chemphyschem : a European journal of chemical physics and physical chemistry. 10(11) |
| ISSN: | 1439-7641 |
| Popis: | Thiol-covered nanostructured gold has been tested as a platform for the preparation of high-area phospholipid bilayer systems suitable for optical and electrochemical sensing. In situ and ex situ Raman spectroscopy and electrochemical measurements are made to study methylene blue (MB) and flavin-adenine dinucleotide (FAD) incorporation into dimyristoylphosphatidylcholine (DMPC) bilayers prepared by vesicle fusion on dithiothreitol (DTT)-covered nanostructured gold. Results show that lipophilic positively charged MB molecules are incorporated in the bilayer reaching the DTT-gold interface. On the other hand, the negatively charged FAD molecules are immobilized at the outer part of the phospholipid bilayer and cannot be electrochemically detected. Our results demonstrate that DTT-covered nanostructured gold provides a suitable high-area platform for phospholipid membranes that are able to separate and sense different kinds of molecules and biomolecules. |
| Databáze: | OpenAIRE |
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