Miniaturized pH biosensors based on electrochemically modified electrodes with biocompatible polymers

Autor: Boris Lakard, Bernard Fahys, Jean Claude Jeannot, Gilles Martin, William Daniau, Laurent Robert, Michel de Labachelerie, Guillaume Herlem
Přispěvatelé: Laboratoire de Chimie des Matériaux et Interfaces (EA 474) (LCMI), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Métrologie des Oscillateurs, Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)
Rok vydání: 2003
Předmět:
Materials science
Polymers
Surface Properties
Potentiometric titration
Biomedical Engineering
Biophysics
Analytical chemistry
chemistry.chemical_element
Biocompatible Materials
02 engineering and technology
Biosensing Techniques
010402 general chemistry
Polypropylenes
01 natural sciences
Sensitivity and Specificity
[SPI.MAT]Engineering Sciences [physics]/Materials
chemistry.chemical_compound
Electrochemistry
Polyethyleneimine
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Electrodes
ComputingMilieux_MISCELLANEOUS
chemistry.chemical_classification
Polyethylenimine
Miniaturization
Reproducibility of Results
General Medicine
Polymer
Equipment Design
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
0104 chemical sciences
Equipment Failure Analysis
chemistry
Chemical engineering
Electrode
Diethylenetriamine
Surface modification
0210 nano-technology
Platinum
Biosensor
Biotechnology
Zdroj: HAL
Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2004, 19, pp.595-606. ⟨10.1016/S0956-5663(03)00270-7⟩
ISSN: 0956-5663
Popis: Potentiometric pH sensors based on linear polyethylenimine (L-PEI) and linear polypropylenimine(L-PPI), two synthetic enzymes and biocompatible polymers, films were prepared by electropolymerization of three different monomers: ethylenediamine (EDA), 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA) in order to be used in clinical, dermatological and biological applications, such as in vivo analysis. In a first step a biosensor was tested which consisted in a platinum wire protruded from glass sheath. The polymer film coated on these platinum electrodes showed good linear potentiometric responses to pH changes from pH 3 to 10. Resulting electrodes present both good reversibility and good stability versus time. The effect of the different polymer film thicknesses to potentiometric responses was also studied. This study allowed us to develop a miniaturized pH biosensor in the second step. This sensor was fabricated using photo-lithography, followed by sputtering and lift-off processes, and it included an electronic detection system. We have also successfully studied the potentiometric responses to pH changes of this device over a period of 1 month, and so we propose this new pH micro-biosensor as an alternative to classical pH sensors currently used in dermatology.
Databáze: OpenAIRE
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