Miniaturized pH biosensors based on electrochemically modified electrodes with biocompatible polymers
Autor: | Boris Lakard, Bernard Fahys, Jean Claude Jeannot, Gilles Martin, William Daniau, Laurent Robert, Michel de Labachelerie, Guillaume Herlem |
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Přispěvatelé: | Laboratoire de Chimie des Matériaux et Interfaces (EA 474) (LCMI), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Métrologie des Oscillateurs, Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) |
Rok vydání: | 2003 |
Předmět: |
Materials science
Polymers Surface Properties Potentiometric titration Biomedical Engineering Biophysics Analytical chemistry chemistry.chemical_element Biocompatible Materials 02 engineering and technology Biosensing Techniques 010402 general chemistry Polypropylenes 01 natural sciences Sensitivity and Specificity [SPI.MAT]Engineering Sciences [physics]/Materials chemistry.chemical_compound Electrochemistry Polyethyleneimine [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics Electrodes ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Polyethylenimine Miniaturization Reproducibility of Results General Medicine Polymer Equipment Design Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology 0104 chemical sciences Equipment Failure Analysis chemistry Chemical engineering Electrode Diethylenetriamine Surface modification 0210 nano-technology Platinum Biosensor Biotechnology |
Zdroj: | HAL Biosensors and Bioelectronics Biosensors and Bioelectronics, Elsevier, 2004, 19, pp.595-606. ⟨10.1016/S0956-5663(03)00270-7⟩ |
ISSN: | 0956-5663 |
Popis: | Potentiometric pH sensors based on linear polyethylenimine (L-PEI) and linear polypropylenimine(L-PPI), two synthetic enzymes and biocompatible polymers, films were prepared by electropolymerization of three different monomers: ethylenediamine (EDA), 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA) in order to be used in clinical, dermatological and biological applications, such as in vivo analysis. In a first step a biosensor was tested which consisted in a platinum wire protruded from glass sheath. The polymer film coated on these platinum electrodes showed good linear potentiometric responses to pH changes from pH 3 to 10. Resulting electrodes present both good reversibility and good stability versus time. The effect of the different polymer film thicknesses to potentiometric responses was also studied. This study allowed us to develop a miniaturized pH biosensor in the second step. This sensor was fabricated using photo-lithography, followed by sputtering and lift-off processes, and it included an electronic detection system. We have also successfully studied the potentiometric responses to pH changes of this device over a period of 1 month, and so we propose this new pH micro-biosensor as an alternative to classical pH sensors currently used in dermatology. |
Databáze: | OpenAIRE |
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