Cloned cDNA to cholecystokinin mRNA predicts an identical preprocholecystokinin in pig brain and gut
| Autor: | Beth J. Hoffman, Kenneth J. Collier, Anne O. Chua, John Eng, Ueli Gubler |
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| Rok vydání: | 1984 |
| Předmět: |
Signal peptide
Duodenum Swine Prohormone Biology Molecular cloning digestive system Intestinal mucosa Complementary DNA medicine Protein biosynthesis Animals RNA Messenger Cloning Molecular Intestinal Mucosa Protein Precursors Cholecystokinin Brain Chemistry chemistry.chemical_classification Multidisciplinary Base Sequence digestive oral and skin physiology DNA Molecular biology Amino acid chemistry Biochemistry Protein Biosynthesis RNA Poly A hormones hormone substitutes and hormone antagonists Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 81:4307-4310 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.81.14.4307 |
| Popis: | Molecular cloning has established the structure of preprocholecystokinin in porcine cerebral cortex and duodenal mucosa. This precursor is 114 amino acids long, is identical in brain and gut, contains all the cholecystokinin (CCK) peptides previously isolated, and has the characteristics of a prohormone. It contains a putative amino-terminal signal peptide, basic processing sites, and a carboxyl-terminal amidation signal. The CCK mRNAs from brain and gut are approximately 850 nucleotides long and differ by only a few single base changes. This analysis establishes by a strict criterion that CCK is synthesized in both brain and gut and that the different distributions of molecular forms of CCK in the two tissues are most probably a consequence of tissue-specific posttranslational events. |
| Databáze: | OpenAIRE |
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