An improved method for the preparation of mycobacterial spheroplasts and the mechanism involved in the reversion to bacillary form: electron microscopic and physiological study
| Autor: | Takezo Udou, Yasuo Mizuguchi, Midori Ogawa |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Cytoplasm
Immunology Reversion Glycine Spheroplasts Applied Microbiology and Biotechnology Microbiology Mycobacterium chemistry.chemical_compound Genetics Magnesium Molecular Biology Budding Bacteriological Techniques biology Mycobacterium smegmatis fungi General Medicine Protoplast Spheroplast biology.organism_classification chemistry Biophysics Microscopy Electron Scanning Calcium Muramidase Lysozyme |
| Zdroj: | Canadian journal of microbiology. 29(1) |
| ISSN: | 0008-4166 |
| Popis: | An efficient method is described for preparing spheroplasts and protoplasts by treating bacillary cells of Mycobacterium smegmatis with precise concentrations of L-glycine (followed by lysozyme). This improved procedure was widely applicable to many rapidly growing mycobacteria by selecting the concentrations of glycine suitable for the individual strains used. The process of reversion of spheroplasts to original bacillary form on solid and in liquid media, as revealed by electron microscopy, appeared to involve the formation of an internal elementary or initial body with subsequent budding from the spheroplast. The internal membrane systems appeared to function in the induction of initial bodies and in the maturation of elementary bodies to become dividing forms. Possible mechanisms involved in the development of bacilli from spheroplasts are discussed. |
| Databáze: | OpenAIRE |
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