Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque
| Autor: | Eo Jin Kim, Soo Min Shon, Myoung Mook Lee, Ju Hee Ryu, Ick Chan Kwon, Jong Ho Park, Jeongyeon Kim, Dong-Eog Kim, Dawid Schellingerhout, Kwangmeyung Kim, Matthias Nahrendorf, Byeong-Teck Kang, Sang Wuk Jeong, Dong Kun Lee |
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| Rok vydání: | 2010 |
| Předmět: |
Apolipoprotein E
Blood Glucose Leptin medicine.medical_specialty Infrared Rays medicine.medical_treatment Aortic Diseases Down-Regulation Physical exercise Matrix metalloproteinase Lipid peroxidation chemistry.chemical_compound Mice Apolipoproteins E In vivo Internal medicine medicine Animals Aorta Mice Knockout Analysis of Variance Microscopy Adiponectin business.industry Body Weight Atherosclerosis Immunohistochemistry Lipids Plaque Atherosclerotic Exercise Therapy Molecular Imaging Disease Models Animal Endocrinology Cytokine chemistry Matrix Metalloproteinase 9 Cytokines Matrix Metalloproteinase 2 Lipid Peroxidation Cardiology and Cardiovascular Medicine business |
| Zdroj: | Atherosclerosis. 216(1) |
| ISSN: | 1879-1484 |
| Popis: | Objective Few studies have investigated if exercise by itself has anti-atherosclerotic effects, without combining interventions with a low-fat diet. We studied the effects of exercise as a stand-alone intervention on preexisting atheromata by measuring not only plaque size but also the levels of plaque-destabilizing matrix-metalloproteinase (MMP) activity in vivo . Methods and results We used near-infrared fluorescent (NIRF) molecular imaging with an MMP-2/9 activatable NIRF probe to visualize the inflammatory protease activity within preexisting atheromata of 17-week-old ApoE −/− mice on: (a) normal chow diet (NCD), (b) Western diet (WD), and (c) WD with treadmill exercise for 10 weeks. We also measured tissue levels of aortic lipid peroxidation (LPO) and plasma levels of glucose/lipid/cytokine profiles. Exercise did not attenuate growth of preexisting atheromatous plaques. However, exercise strongly decreased proteolytic activity in plaques for animals on WD, with levels decreasing almost to NCD levels. Exercise was associated with decreased aortic LPO levels and increased blood adiponectin/leptin levels; however, exercise did not affect WD-consumption/weight-gain or improve blood glucose/lipid profiles. Conclusions Exercise training reduced aortic MMP activity in mice with preexisting atheromata, even though they remained on a high fat diet and plaque-growth was not attenuated. |
| Databáze: | OpenAIRE |
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