Cellulase enhances endophytism of encapsulated Metarhizium brunneum in potato plants
| Autor: | Vivien Krell, Stefan Vidal, Anant V. Patel, Desirée Jakobs-Schoenwandt |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Metarhizium Biological pest control penetration biological control Cellulase Biology 01 natural sciences Endophyte 660.6 03 medical and health sciences chemistry.chemical_compound Genetics Endophytes Food science Cellulose Ecology Evolution Behavior and Systematics Mycelium Solanum tuberosum 2. Zero hunger fungi food and beverages Spores Fungal biology.organism_classification Yeast 030104 developmental biology Infectious Diseases chemistry comic_books Metarhizium brunneum biology.protein encapsulation endophyte comic_books.character 010606 plant biology & botany |
| Zdroj: | Fungal biology. 122(5) |
| ISSN: | 1878-6146 |
| Popis: | The recent discovery that entomopathogenic fungi can grow endophytically in plant tissues has spurred research into novel plant protection measures. However, current applications of fungi aiming at endophytism mostly lack targeted formulation strategies resulting in low efficacy. Here, we aimed at enhancing Metarhizium brunneum CB15 endophytism in potato plants by (i) improvement of fungal growth from beads and (ii) cellulase formation or addition to encapsulated mycelium. We found that beads supplemented with cellulose alone or in addition with inactivated baker's yeast exhibited cellulase activity and increased mycelial growth by 12.6 % and 13.6 %, respectively. Higher enzymatic activity achieved by cellulase co-encapsulation promoted a shift from mycelial growth to spore formation with maximum numbers of 2.5 × 108 ± 6.1 × 107 per bead. This correlated with improved endophytism in potato plants by 61.2 % compared to non-supplemented beads. Our study provides first evidence that customized formulations of fungal entomopathogens with enzymes can improve endophytism and this may increase efficacy in plant protection strategies against herbivorous pests. |
| Databáze: | OpenAIRE |
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