The stacking of chloroplast thylakoids
| Autor: | S. W. Thorne, N.K. Boardman, W.S. Chow, J.T. Duniec, M.J. Sculley |
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| Rok vydání: | 1980 |
| Předmět: |
Cation binding
Sulfolipid Biophysics Stacking Phospholipid macromolecular substances Biochemistry Divalent symbols.namesake chemistry.chemical_compound Ion binding polycyclic compounds Molecular Biology Differential centrifugation chemistry.chemical_classification Chromatography Chemistry food and beverages Chloroplast Digitonin Membrane Thylakoid symbols van der Waals force |
| Zdroj: | Archives of Biochemistry and Biophysics. 201:339-346 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(80)90519-6 |
| Popis: | The differential action of digitonin on stacked and unstacked chloroplast thylakoids was used to investigate the molecular interactions between thylakoid membranes. The yield of the heavy fraction which is obtained from chloroplasts after digitonin incubation and differential centrifugation was taken as a measure of the degree or tightness of membrane appression. The effects of various mono-, di-, and trivalent cations on the yield of the heavy fraction were studied, and the results interpreted in terms either of electrostatic screening or ion binding to the thylakoid membrane surface: Although there was some degree of cation specificity in the degree of thylakoid appression indicative of cation binding, the nonspecific screening effect was much more important in determining the overall balance of forces. It is postulated that stacking occurs in regions of low net surface charge density, with a possible segregation of excess negative charges into nonstacked regions. |
| Databáze: | OpenAIRE |
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