Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone

Autor: Tetsuya Yamada, Hidemasa Nakaminami, Yuka Maezawa, Hideaki Ikoshi, Keisuke Nakase, Norihisa Noguchi, Shunsuke Takadama, Minami Hayashi, Takeaki Wajima
Rok vydání: 2019
Předmět:
0301 basic medicine
Staphylococcus
Skin flora
Leukocidin
Gene Expression
Pathology and Laboratory Medicine
medicine.disease_cause
Leukocidins
Staphylococcus epidermidis
Medicine and Health Sciences
Staphylococcus Aureus
skin and connective tissue diseases
Multidisciplinary
Antimicrobial
Anti-Bacterial Agents
Bacterial Pathogens
Infectious Diseases
Medical Microbiology
Staphylococcus aureus
Medicine
Pathogens
Research Article
Methicillin-Resistant Staphylococcus aureus
Skin Infections
Science
Bacterial Toxins
030106 microbiology
Exotoxins
Virulence
Dermatology
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Complementary and Alternative Medicine
Genetics
medicine
Humans
Staphylococcus Epidermidis
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Bacteria
Organisms
Biology and Life Sciences
Gene Expression Regulation
Bacterial
biochemical phenomena
metabolism
and nutrition
Traditional Medicine
bacterial infections and mycoses
biology.organism_classification
Methicillin-resistant Staphylococcus aureus
030104 developmental biology
Medicine
Kampo
Panton–Valentine leukocidin
Cloning
Zdroj: PLoS ONE, Vol 14, Iss 3, p e0214470 (2019)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0214470
Popis: It is necessary to develop agents other than antimicrobials for the treatment of Staphylococcus aureus infections to prevent the emergence of antimicrobial-resistant strains. Particularly, anti-virulence agents against the Panton-Valentine leukocidin (PVL)-positive methicillin-resistant S. aureus (MRSA), USA300 clone, is desired due to its high pathogenicity. Here, we investigated the potential anti-virulence effect of Tokiinshi, which is a traditional Japanese medicine (Kampo) used for skin diseases, against the USA300 clone. A growth inhibition assay showed that a conventional dose (20 mg/ml) of Tokiinshi has bactericidal effects against the clinical USA300 clones. Notably, the growth inhibition effects of Tokiinshi against S. epidermidis strains, which are the major constituents of the skin microbiome, was a bacteriostatic effect. The data suggested that Tokiinshi is unlikely to affect skin flora of S. epidermidis. Furthermore, PVL production and the expression of its gene were significantly suppressed in the USA300 clone by a lower concentration (5 mg/ml) of Tokiinshi. This did not affect the number of viable bacteria. Moreover, Tokiinshi significantly suppressed the expression of the agrA gene, which regulates PVL gene expression. For the first time, our findings strongly suggest that Tokiinshi has the potential to attenuate the virulence of the USA300 clone by suppressing PVL production via agrA gene suppression.
Databáze: OpenAIRE
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