Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics
| Autor: | Clara Marin, Jose S. Vicente, Francisco Marco-Jiménez, María Pilar Viudes-de-Castro |
|---|---|
| Přispěvatelé: | UCH. Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Producción Científica UCH 2021 |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
Veterinary medicine Antibiotics L73 Animal diseases Conejos - Inseminación artificial Bactericidas Spermatozoa - Quality control Ethylenediaminetetraacetic acid Semen Artificial insemination L70 Veterinary science and hygiene Bacterial growth PRODUCCION ANIMAL Article Semen collection 03 medical and health sciences Semen quality chemistry.chemical_compound fluids and secretions SF600-1100 medicine Food science 030304 developmental biology Antibióticos en veterinaria 0303 health sciences Germicides General Veterinary Chemistry 0402 animal and dairy science Antibiotics in veterinary medicine 04 agricultural and veterinary sciences Esperma - Control de calidad 040201 dairy & animal science Semen extender Reproductive performance Rabbits - Artificial insemination QL1-991 Animal Science and Zoology Antibacterial activity Zoology |
| Zdroj: | Animals, Vol 11, Iss 1178, p 1178 (2021) CEU Repositorio Institucional Fundación Universitaria San Pablo CEU (FUSPCEU) electronico ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias Universidad de Alicante (UA) Animals : an Open Access Journal from MDPI RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname ReDivia: Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias Instituto Valenciano de Investigaciones Agrarias (IVIA) Animals Volume 11 Issue 4 |
| DOI: | 10.3390/ani11041178 |
| Popis: | [EN] This study was conducted to evaluate the antibacterial activity of two aminopeptidase inhibitors and chitosan-based nanoparticles in liquid-stored rabbit semen. This study reports that the aminopeptidase inhibitors used to prevent bacterial growth could be used in semen extender as a suitable alternative to antibiotics. Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 degrees C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenders. Funding from the Ministry of Economy, Industry and Competitiveness (Research project: AGL2017-85162-C2-1-R) is acknowledged. |
| Databáze: | OpenAIRE |
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