Ethylchloroformate Derivatization for GC–MS Analysis of Resveratrol Isomers in Red Wine
| Autor: | Alessandra Bonamore, Federica Palombarini, Alessio Incocciati, Elisa Di Fabio, Alberto Macone, Alberto Boffi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Formic Acid Esters
Pharmaceutical Science Wine ethylchloroformate resveratrol Resveratrol 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Drug Discovery gas chromatography–mass spectrometry gas chromatography-mass spectrometry red wine Physical and Theoretical Chemistry Derivatization Beneficial effects Chromatography Ethylchloroformate 010405 organic chemistry Natural compound 010401 analytical chemistry Organic Chemistry Reproducibility of Results Quantitative determination 0104 chemical sciences chemistry Chemistry (miscellaneous) Molecular Medicine Gas chromatography–mass spectrometry |
| Zdroj: | Molecules Volume 25 Issue 20 Molecules, Vol 25, Iss 4603, p 4603 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25204603 |
| Popis: | Resveratrol (3,5,4&prime trihydroxystilbene) is a natural compound that can be found in high concentrations in red wine and in many typical foods found in human diet. Over the past decades, resveratrol has been widely investigated for its potential beneficial effects on human health. At the same time, numerous analytical methods have been developed for the quantitative determination of resveratrol isomers in oenological and food matrices. In the present work, we developed a very fast and sensitive GC&ndash MS method for the determination of resveratrol in red wine based on ethylchloroformate derivatization. Since this reaction occurs directly in the water phase during the extraction process itself, it has the advantage of significantly reducing the overall processing time for the sample. This method presents low limits of quantification (LOQ) (25 ng/mL and 50 ng/mL for cis- and trans-resveratrol, respectively) and excellent accuracy and precision. Ethylchloroformate derivatization was successfully applied to the analysis of resveratrol isomers in a selection of 15 commercial Italian red wines, providing concentration values comparable to those reported in other studies. As this method can be easily extended to other classes of molecules present in red wine, it allows further development of new GC&ndash MS methods for the molecular profiling of oenological matrices. |
| Databáze: | OpenAIRE |
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