The DNA methyltransferase inhibitor zebularine exerts antitumor effects and reveals BATF2 as a poor prognostic marker for childhood medulloblastoma

Autor: Angel Mauricio Castro-Gamero, Luciano Neder, Augusto Faria Andrade, Lenisa Geron, Veridiana Kiill Suazo, Carolina Alves Pereira Corrêa, Simone dos Santos Aguiar, Kleiton Silva Borges, Elton J R Vasconcelos, José Andrés Yunes, Carlos Alberto Scrideli, Ricardo Santos de Oliveira, Luiz Gonzaga Tone
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Cyclin A
DNA Methyltransferase Inhibitor
Apoptosis
Cell Cycle Proteins
Cytidine
chemistry.chemical_compound
0302 clinical medicine
Medicine
Drug Interactions
Pharmacology (medical)
Child
DNA Modification Methylases
Oligonucleotide Array Sequence Analysis
biology
Prognosis
Gene Expression Regulation
Neoplastic
Basic-Leucine Zipper Transcription Factors
Oncology
Vincristine
Child
Preschool
030220 oncology & carcinogenesis
DNA methylation
Female
medicine.drug
Adult
Adolescent
Antineoplastic Agents
DNA methyltransferase
Young Adult
03 medical and health sciences
Cell Line
Tumor
Survivin
Humans
Cerebellar Neoplasms
Pharmacology
Cisplatin
Medulloblastoma
ANTINEOPLÁSICOS
business.industry
Tumor Suppressor Proteins
Infant
Newborn
Infant
medicine.disease
030104 developmental biology
Zebularine
chemistry
Cancer research
biology.protein
business
Biomarkers
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1573-0646
0167-6997
DOI: 10.1007/s10637-016-0401-4
Popis: Medulloblastoma (MB) is the most common solid tumor among pediatric patients and corresponds to 20 % of all pediatric intracranial tumors in this age group. Its treatment currently involves significant side effects. Epigenetic changes such as DNA methylation may contribute to its development and progression. DNA methyltransferase (DNMT) inhibitors have shown promising anticancer effects. The agent Zebularine acts as an inhibitor of DNA methylation and shows low toxicity and high efficacy, being a promising adjuvant agent for anti-cancer chemotherapy. Several studies have reported its effects on different types of tumors; however, there are no studies reporting its effects on MB. We analyzed its potential anticancer effects in four pediatric MB cell lines. The treatment inhibited proliferation and clonogenicity, increased the apoptosis rate and the number of cells in the S phase (p
Databáze: OpenAIRE
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