EFFECT OF RIBONUCLEASE ON THE INFECTIVITY OF HEATED ARBOVIRUSES
| Autor: | Vlastimil Mayer |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Infectivity
Deoxyribonucleases Hot Temperature biology Chemical Phenomena viruses Research Clone (cell biology) General Medicine DNA Virology General Biochemistry Genetics and Molecular Biology Virus Microbiology Encephalitis Viruses Chemistry Ribonucleases Viral envelope Capsid biology.protein Denaturation (biochemistry) Ribonuclease General Pharmacology Toxicology and Pharmaceutics Arboviruses |
| Zdroj: | Life sciences (1962). 3 |
| ISSN: | 0161-5564 |
| Popis: | Changes in infectivity represent the most sensitive indicator of the effect of high temperatures on the biological activity of virus particles (1). The infectivity of virus during the process of thermal inactivation is influenced both by denaturation of surface structural groups of the virus, preventing effective adsorption on the host cell, and by the degradation of the viral nucleic acid. Structural changes of the capsid occuring during thermal inactivation are little known, particularly in arboviruses. We assumed that higher temperatures might injure the viral envelope in such a way that the inner contents of the particle become more accessible to the action of ribonuclease (RNA-se). We therefore studied the effect of RNA-se on the infectivity of different clones of viruses belonging to the tick-borne encephalitis (TE) complex (subgroup B of arboviruses), and on a clone of Western equine encephalomyelitis (WEE) virus (subgroup A of arboviruses), which had been heated for a short time at 50° or 60° C. |
| Databáze: | OpenAIRE |
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