Surface Colonization and Activity of the 2,6-Dichlorobenzamide (BAM) Degrading Aminobacter sp. Strain MSH1 at Macro- and Micropollutant BAM Concentrations
| Autor: | Johan Hofkens, Julie Vanden Bussche, Lynn Vanhaecke, Aswini Sekhar, Benjamin Horemans, Dirk Springael, Sebastian R. Sørensen, Jens Aamand |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Portable water purification 010501 environmental sciences 01 natural sciences Mineralization (biology) Water Purification Microbiology 03 medical and health sciences Environmental Chemistry Groundwater 0105 earth and related environmental sciences 2. Zero hunger Pollutant Chemistry Biofilm food and beverages Phyllobacteriaceae General Chemistry Biodegradation 6. Clean water Biodegradation Environmental 13. Climate action Environmental chemistry Soil water Degradation (geology) Water treatment Filtration |
| Zdroj: | Environmental Science & Technology. 50:10123-10133 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.6b01978 |
| Popis: | Aminobacter sp. MSH1 uses the groundwater micropollutant 2,6-dichlorobenzamide (BAM) as a C and N source and is a potential catalyst for biotreatment of BAM-contaminated groundwater in filtration units of drinking water treatment plants (DWTPs). The oligotrophic environment of DWTPs including trace pollutant concentrations, and the high flow rates impose challenges for micropollutant biodegradation in DWTPs. To understand how trace BAM concentrations affect MSH1 surface colonization and BAM degrading activity, MSH1 was cultivated in flow channels fed continuously with BAM macro- and microconcentrations in a N- and C-limiting medium. At all BAM concentrations, MSH1 colonized the flow channel. BAM degradation efficiencies were concentration-dependent, ranging between 70 and 95%. Similarly, BAM concentration affected surface colonization, but at 100 μg/L BAM and lower, colonization was similar to that in systems without BAM, suggesting that assimilable organic carbon and nitrogen other than those supplied by BAM sustained colonization at BAM microconcentrations. Comparison of specific BAM degradation rates in flow channels and in cultures of suspended freshly grown cells indicated that starvation conditions in flow channels receiving BAM microconcentrations resulted into MSH1 biomasses with 10-100-times reduced BAM degrading activity and provided a kinetic model for predicting BAM degradation under continuous C and N starvation. |
| Databáze: | OpenAIRE |
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