Developing human lung and kidney are major sites for synthesis of bone morphogenetic protein-3 (osteogenin)
| Autor: | Slobodan Vukicevic, Frank P. Luyten, M N Helder |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
medicine.medical_specialty animal structures Histology Bone Morphogenetic Protein 3 In situ hybridization Kidney Bone morphogenetic protein 3 Bone morphogenetic protein Embryonic and Fetal Development Intestinal mucosa Internal medicine medicine Humans Perichondrium RNA Messenger Northern blot Lung In Situ Hybridization biology RNA Probes Immunohistochemistry Molecular biology Bone morphogenetic protein 7 Endocrinology Protein Biosynthesis Bone Morphogenetic Proteins embryonic structures biology.protein Anatomy Immunostaining |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 42:869-875 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/42.7.8014470 |
| Popis: | Bone morphogenetic protein-3 (BMP-3; osteogenin) is a member of the transforming growth factor-beta superfamily. We used human BMP-3 cDNA probes and a specific BMP-3 polyclonal peptide antibody to analyze BMP-3 expression and synthesis in human fetal and adult tissues. Northern blot hybridization revealed two mRNA species of 7 and 3 KB. High levels of BMP-3 mRNA were found in fetal lungs. By in situ hybridization, the BMP-3 transcripts were found in lung bronchial epithelium, straight collecting kidney tubules, intestinal mucosa, perichondrium, periosteum, and osteoblasts of human embryos of 6-14 weeks' gestation. Cellular BMP-3 immunostaining co-localized with the distribution of RNA. In addition, bone matrix showed intensive BMP-3 staining. These data suggest that although BMP-3 has been isolated from bone matrix, it may have additional regulatory roles in the morphogenesis and/or function of human lung, kidney, and intestine. |
| Databáze: | OpenAIRE |
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