Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines
Autor: | Gustavo Marçal Schmidt Garcia Moreira, Emili Griep, Fabricio Rochedo Conceição, Rafael R. Rodrigues, Lucas Moreira dos Santos, Rafael Amaral Donassolo, Clóvis Moreira, Marcos Roberto Alves Ferreira, Ângela Nunes Moreira |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Kanamycin Resistance Gene Transfer Horizontal animal diseases Microorganism Formaldehyde Bioengineering complex mixtures 01 natural sciences Applied Microbiology and Biotechnology Microbiology law.invention 03 medical and health sciences chemistry.chemical_compound Plasmid Clostridium law 010608 biotechnology Escherichia coli Gene Vaccines Synthetic biology Chemistry Escherichia coli Vaccines Electroporation General Medicine biology.organism_classification 030104 developmental biology Vaccines Inactivated Recombinant DNA Biotechnology Plasmids |
Zdroj: | Biotechnology letters. 42(11) |
ISSN: | 1573-6776 |
Popis: | Earlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. These bacterins have a simpler, safer, and faster production process. However, these bacterins carry expression plasmids, containing antibiotic resistance gene, which could be assimilate accidentally by environmental microorganisms. Considering this, we aimed to impair this plasmids using formaldehyde at different concentrations. This compound inactivated the highest density of cells in 24 h. KanR cassette amplification was found to be impaired with 0.8% for 24 h or 0.4% for 72 h. Upon electroporation, E. coli DH5α ultracompetent cells were unable to acquire the plasmids extracted from the bacterins after inactivation procedure. Formaldehyde-treated bacterins were incubated with other viable strains of E. coli, leading to no detectable gene transfer. We found that this compound is effective as an inactivation agent. Here we demonstrate the biosafety involving antibiotic resistance gene of recombinant E. coli vaccines allowing to industrial production and animal application. |
Databáze: | OpenAIRE |
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