Association of tumor necrosis factor-α gene polymorphism with osteoarticular tuberculosis prognosis in a Hebei population
Autor: | W.N. Hu, R.Q. Li, S.J. Liu, Z.K. Zhang, L.D. Zheng, Y.C. Zhang, Q.Y. Ren, G.P. Zhang, Y.J. Lv |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Genotype Population Gastroenterology Polymorphism Single Nucleotide Tuberculosis Osteoarticular 03 medical and health sciences 0302 clinical medicine Risk Factors Internal medicine Genetics Medicine Humans Genetic Predisposition to Disease Allele education Molecular Biology Allele frequency Alleles Genetic Association Studies Genetic association education.field_of_study business.industry Tumor Necrosis Factor-alpha Haplotype General Medicine Venous blood Middle Aged Prognosis 030104 developmental biology Haplotypes 030220 oncology & carcinogenesis Immunology Female Gene polymorphism business |
Zdroj: | Genetics and molecular research : GMR. 15(4) |
ISSN: | 1676-5680 |
Popis: | This study investigated the association of tumor necrosis factor-α (TNF-α)-308, -238, and -863 polymorphisms with osteoarticular tuberculosis (OA-TB) prognosis in a Hebei population. Genomic DNA was extracted from venous blood samples of 120 OA-TB patients and 100 healthy volunteers. TNF-α-308, -238, and -863 were analyzed by PCR-restriction fragment length polymorphism; genotype and allele frequencies were calculated. Serum TNF-α level was significantly higher in OA-TB patients (283.16 ± 51.68 ng/L) than in control (122.54 ± 54.65 ng/L; P < 0.05). Higher frequency of TNF-α-308 GG genotype in healthy volunteers (91.0%) than in OA-TB patients (79.2%) indicated that it was a protective factor against OA-TB (OR = 0.405, 95%CI = 0.147-0.657, P = 0.007). Higher frequencies of TNF-α-308 GA genotype and TNF-α-308 allele (A) in OA-TB patients (20.8 and 10.4%, respectively) than in healthy volunteers (8.0 and 5.0%, respectively) indicated an association with increased risk of OA-TB (OR = 3.112, 95%CI = 1.520-6.343, P = 0.003; OR = 3.109, 95%CI = 1.676-6.538, P = 0.006; respectively). Haplotype association analysis of TNF-α polymorphisms (-308/-238/-863) showed a higher frequency of TNF-α AGA in OA-TB patients (12.1%) than in healthy volunteers (3.5%), indicating that it was a risk factor for OA-TB (OR = 4.201, 95%CI = 1.80-9.91, P = 0.010). TNF-α-308 G/A and TNF-α AGA (-308/-238/-863) were associated with a predisposition to OA-TB, which could aid clinical detection, prevention, and prognosis of OA-TB. |
Databáze: | OpenAIRE |
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