Ionizing radiation modulates vascular endothelial growth factor expression through STAT3 signaling pathway in rat neonatal primary astrocyte cultures
Autor: | Yilin Wang, Yunqian Chang, Bing He, Guijuan Zhou, Jianghua Huang, Zijian Xiao, Yangzhi Xie, Yan Xu, Rundong Ma, Lin Wu |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
STAT3 Transcription Factor
Vascular Endothelial Growth Factor A medicine.medical_treatment 050105 experimental psychology Stat3 Signaling Pathway lcsh:RC321-571 Rats Sprague-Dawley 03 medical and health sciences Behavioral Neuroscience chemistry.chemical_compound 0302 clinical medicine Radiation Ionizing Animals Medicine 0501 psychology and cognitive sciences STAT3 radiation‐induced brain injury lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research Cerebral Cortex Glial fibrillary acidic protein biology vascular endothelial growth factor business.industry 05 social sciences astrocytes Rats Vascular endothelial growth factor medicine.anatomical_structure Cytokine chemistry glial fibrillary acidic protein biology.protein Cancer research STAT protein signal transducer and activator of transcription 3 Signal transduction business 030217 neurology & neurosurgery Signal Transduction Astrocyte |
Zdroj: | Brain and Behavior, Vol 10, Iss 4, Pp n/a-n/a (2020) Brain and Behavior |
ISSN: | 2162-3279 |
Popis: | Background and Purpose Radiation‐induced brain injury (RBI) usually occurs six months to three years after irradiation, often shows cognitive dysfunction, epilepsy, and other neurological dysfunction. In severe cases, it can cause a wide range of cerebral edema, even herniation. It seriously threatens the survival of patients and their quality of life, and it becomes a key factor in limiting the radiation dose and lowering the therapeutic efficacy in recent years. Therefore, studying the pathogenesis of RBI and exploring new therapeutic targets are of great significance. Methods In our study, we observed the activation and secretory function in astrocytes as well as the intracellular signal transducer and activator of transcription 3 (STAT3) signal transduction pathway activation status after exposing different doses of X‐ray irradiation by using MTT, Immunocytologic analysis, and Western blot analysis. Further, we used the same way to explore the role of vascular endothelial growth factor (VEGF) in signal transduction pathways playing in the activation of astrocytes after irradiating through the use of specificInhivascular endothelial growth factorbitors of STAT3. Results Ast can be directly activated, reactive hyperplasia and hypertrophy, the expression of the activation marker glial fibrillary acidic protein is increased, and the expression of vascular endothelial growth factor (VEGF) in the cells is increased, which may lead to RBI. After the addition of STAT3 pathway inhibitor, most of the Ast radiation activation was suppressed, and the expression of high‐level expression of VEGF decreased after irradiation. Conclusion Our findings demonstrated that X‐ray irradiation directly induced the activation of astrocytes in a persistent manner and X‐ray irradiation activated STAT3 signaling pathway. As the same time, we found that X‐ray irradiation induced the activation of astrocytes and secretion cytokine. The STAT3 signaling pathway may participate in the pathogenesis of radiation‐induced brain injury. In this work, we found the changes of vascular endothelial growth factor(VEGF) and glial fibrillary acidic protein (GFAP), and the signal transducer and activator of transcription 3(STAT3) signaling pathways involved in astrocytes exposure to X‐rays may be instructive for improving the efficiency of radiation therapy while reducing damage to normal brain tissue. |
Databáze: | OpenAIRE |
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