Human Alveolar Macrophages May Not Be Susceptible to Direct Infection by a Human Influenza Virus
| Autor: | Norbert J. Roberts, Mark W. Frampton, David B. Ettensohn, Joan E. Nichols |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine media_common.quotation_subject Virus Attachment Biology Antibodies Viral Virus Replication medicine.disease_cause Virus Microbiology Young Adult Major Articles and Brief Reports 03 medical and health sciences 0302 clinical medicine Influenza Human Macrophages Alveolar Influenza A virus medicine Humans Immunology and Allergy Macrophage Internalization Cells Cultured media_common RNA Virus Internalization Virology In vitro Viral Tropism 030104 developmental biology Infectious Diseases biology.protein Female Antibody Neuraminidase 030215 immunology |
| Zdroj: | Journal of Infectious Diseases. 214:1658-1665 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiw413 |
| Popis: | The current studies were undertaken to determine the susceptibility of human alveolar macrophages (AMs) to influenza A virus (IAV) infection in comparison with autologous peripheral blood–derived monocytes-macrophages (PBMs). AMs and PBMs were exposed to IAV in vitro and examined for their ability to bind and internalize IAV, and synthesize viral proteins and RNA. PBMs but not AMs demonstrated binding and internalization of the virus, synthesizing viral proteins and RNA. Exposure of AMs in the presence of a sialidase inhibitor or anti-IAV antibody resulted in viral protein synthesis by the cells. Exposure of AMs to fluorescein isothiocyanate–labeled IAV in the presence of anti–fluorescein isothiocyanate antibody also resulted in viral protein synthesis. Thus, human AMs are apparently not susceptible to direct infection by a human IAV but are likely to be infected indirectly in the setting of exposure in the presence of antibody that binds the challenging strain of IAV. |
| Databáze: | OpenAIRE |
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