Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity
| Autor: | Juha A. E. Määttä, Vesa P. Hytönen, Niklas Kähkönen, Markku S. Kulomaa, Soili I. Lehtonen, Hermann J. Gruber, Teemu O. Ihalainen, Masi Koskinen, Chloe Thomson, Sampo Kukkurainen, Dominik Zauner, Barbara Taskinen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Mutant
Biomedical Engineering Pharmaceutical Science Biotin Bioengineering Biosensing Techniques Conjugated system chemistry.chemical_compound Mice Animals Biotinylation Surface plasmon resonance Binding site Pharmacology Binding Sites biology Calorimetry Differential Scanning Chemistry Organic Chemistry 3T3 Cells Surface Plasmon Resonance Avidin 3. Good health Biochemistry Mutation biology.protein Biosensor Chickens Biotechnology |
| Zdroj: | Bioconjugate chemistry. 25(12) |
| ISSN: | 1520-4812 |
| Popis: | Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|