Effect of 8-isoprostaglandin F2α on the newborn rat pulmonary arterial muscle and endothelium
| Autor: | C. R. Pace-Asciak, J. Belik, A. K. Tanswell, Robert P. Jankov, Man Yi, Jingyi Pan |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Antioxidant Endothelium Physiology medicine.medical_treatment Pulmonary Artery Muscle Smooth Vascular Rats Sprague-Dawley Physiology (medical) Internal medicine medicine Animals Vasoconstrictor Agents Enzyme Inhibitors Respiratory system chemistry.chemical_classification Prostaglandins A Reactive oxygen species Lung biology Age Factors Anatomy Isoprostanes Endothelin 1 Rats Vasodilation NG-Nitroarginine Methyl Ester Endocrinology medicine.anatomical_structure Animals Newborn chemistry Vasoconstriction 15-Hydroxy-11 alpha 9 alpha-(epoxymethano)prosta-5 13-dienoic Acid biology.protein Endothelium Vascular Cyclooxygenase |
| Zdroj: | Journal of Applied Physiology. 95:1979-1985 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.00420.2003 |
| Popis: | 8-Isoprostaglandin F2α (8-iso-PGF2α) is a bioactive lipid peroxidation product that is a vasoconstrictor at high concentrations. Paradoxically, at lower, and possibly physiological, concentrations, it is a pulmonary vascular muscle's relaxant. Its effects on newborn pulmonary vasculature are unknown. We hypothesized that the pulmonary arterial 8-iso-PGF2α responses may be developmentally regulated. Therefore, the purpose of this study was to evaluate and compare 8-iso-PGF2α effects between 1- and 2-wk-old newborn and adult rat isolated intrapulmonary arteries (100 μm) mounted on a myograph. Force after 8-iso-PGF2α stimulation was greatest in the adult ( P < 0.01). In newborns, force was significantly increased by the nitric oxide (NO) synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME) ( P < 0.01) and was suppressed by blockade of the thromboxane (Tx) A2 receptor. Whereas 8-iso-PGF2α induced a significant dose-dependent relaxation of adult precontracted vessels in the presence of a TxA2 mimetic (U-46619; 1 μM), contraction was observed in the 1-wk-old rat. This 8-iso-PGF2α-induced contraction was abolished by endothelium removal and l-NAME and was attenuated by the cyclooxygenase inhibitor ibuprofen. In the presence of a TxA2/prostaglandin H2 receptor blocker, 8-iso-PGF2α induced NO-mediated relaxation, the magnitude of which was greater in the newborn, compared with the adult ( P < 0.01). When exposed to 8-iso-PGF2α in vitro, only the newborn lung secreted TxB2. We conclude that, in contrast to its relaxant effect in the adult, 8-iso-PGF2α induces contraction of the pulmonary arteries in the early postnatal period, which is likely to be mediated by endothelium-derived TxA2. This phenomenon may contribute to the maintenance of a higher pulmonary vascular resistance in the early postnatal period. |
| Databáze: | OpenAIRE |
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