Plasmodium falciparum Genotype Diversity in Artemisinin Derivatives Treatment Failure Patients along the Thai-Myanmar Border
| Autor: | Thirasak Hoonchaiyapoom, Kornnarin Inorn, Kanungnit Congpuong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Genotype Plasmodium falciparum Protozoan Proteins glurp Antigens Protozoan Myanmar Biology Polymerase Chain Reaction Antimalarials Gene Frequency Genetic variation parasitic diseases medicine Humans Treatment Failure Allele Artemisinin Malaria Falciparum Genotyping Allele frequency Merozoite Surface Protein 1 msp-1 msp-2 Genetic Variation biology.organism_classification medicine.disease Thailand Virology Artemisinins Infectious Diseases Immunology Parasitology Original Article Female Malaria medicine.drug |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| Popis: | Genetic characteristics of Plasmodium falciparum may play a role in the treatment outcome of malaria infection. We have studied the association between diversity at the merozoite surface protein-1 (msp-1), msp-2, and glutamate-rich protein (glurp) loci and the treatment outcome of uncomplicated falciparum malaria patients along the Thai-Myanmar border who were treated with artemisinin derivatives combination therapy. P. falciparum isolates were collected prior to treatment from 3 groups of patients; 50 cases of treatment failures, 50 recrudescences, and 56 successful treatments. Genotyping of the 3 polymorphic markers was analyzed by nested PCR. The distribution of msp-1 alleles was significantly different among the 3 groups of patients but not the msp-2 and glurp alleles. The allelic frequencies of K1 and MAD20 alleles of msp1 gene were higher while RO33 allele was significantly lower in the successful treatment group. Treatment failure samples had a higher median number of alleles as compared to the successful treatment group. Specific genotypes of msp-1, msp-2, and glurp were significantly associated with the treatment outcomes. Three allelic size variants were significantly higher among the isolates from the treatment failure groups, i.e., K1270-290, 3D7610-630, G650-690, while 2 variants, K1150-170, and 3D7670-690 were significantly lower. In conclusion, the present study reports the differences in multiplicity of infection and distribution of specific alleles of msp-1, msp-2, and glurp genes in P. falciparum isolates obtained from treatment failure and successful treatment patients following artemisinin derivatives combination therapy. |
| Databáze: | OpenAIRE |
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