Production of the polyclonal antibody against Sudan 3 and Immunoassay of Sudan dyes in food samples
| Autor: | Wen Chong Shan, Yan Zheng Liu, Yong Hua Qi, Yu Jie Zhang, Jian Ping Wang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Immunogen
Enzyme-Linked Immunosorbent Assay Food Contamination Sudan Red G Antibodies chemistry.chemical_compound Antibody Specificity parasitic diseases medicine Animals Bovine serum albumin Coloring Agents Detection limit Chromatography biology medicine.diagnostic_test General Chemistry chemistry Polyclonal antibodies Immunoassay biology.protein Para Red Carcinogens Rabbits General Agricultural and Biological Sciences Hapten Azo Compounds Haptens |
| Zdroj: | Journal of agricultural and food chemistry. 60(9) |
| ISSN: | 1520-5118 |
| Popis: | In this study, 4-aminophenylacetic acid was covalently coupled to aniline to synthesize an intermediate hapten and the intermediate hapten was coupled to β-naphthol to synthesize a tentative hapten of Sudan 3. The hapten was coupled to bovine serum albumin as the immunogen to produce the polyclonal antibody. The obtained antibody was highly specific to Sudan 3, Sudan 1, and Para red, but showed relative low binding ability to Sudan 2, Sudan 4, and Sudan red G. After evaluation of different coating antigens, a heterologous indirect competitive immunoassay was developed to multidetermine the six red dyes in food samples. The cross reactivities to the six analytes were in a range of 21-105%, and the limits of detection were in a range of 0.1-0.8 ng/mL depending on the compound. Intra- and interassay recoveries from the standard fortified blank samples were in a range of 74.5-96.3% with coefficients of variation lower than 15.1%. |
| Databáze: | OpenAIRE |
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