Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage
| Autor: | Wei-wei Xu, Yu-chao Xu, Xilin Hou, Shi-lin Zhang, Shan-wu Lü, Lu-lu Shen, Chun-mei Hu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Subfamily Agriculture (General) Mutant Stamen Plant Science Biology non-heading Chinese cabbage 01 natural sciences Biochemistry ethylene-responsive factor Homology (biology) S1-972 03 medical and health sciences Food Animals Complementary DNA Brassica rapa Botany Gene Genetics Ecology Software maintainer fungi stamen-petalody food and beverages 030104 developmental biology gene expression Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 15, Iss 3, Pp 528-536 (2016) |
| ISSN: | 2095-3119 |
| Popis: | BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What's more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses. |
| Databáze: | OpenAIRE |
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