Effect of IL-11 on glomerular expression of TGF-beta and extracellular matrix in nephrotoxic nephritis in Wistar Kyoto rats
| Autor: | Maria Stangou, Ping-Chin Lai, Gurjeet Bhangal, Frederick W.K. Tam, Jennifer A. Smith, James C. Keith, Joseph J. Boyle, Charles D. Pusey, Terence Cook |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Kidney Glomerulus Rats Inbred WKY p38 Mitogen-Activated Protein Kinases Nephrotoxicity Transforming Growth Factor beta1 Pathogenesis Glomerulonephritis In vivo Internal medicine TGF beta signaling pathway medicine Animals Humans Phosphorylation Autoantibodies Extracellular Matrix Proteins Kidney biology business.industry Interleukin-11 medicine.disease Actins Recombinant Proteins Fibronectins Rats Fibronectin Disease Models Animal medicine.anatomical_structure Endocrinology Nephrology biology.protein business Nephritis |
| Zdroj: | Journal of Nephrology. 24:106-111 |
| ISSN: | 1121-8428 |
| Popis: | BACKGROUND The effect of interleukin-11 (IL-11) on transforming growth factor-s (TGF-s) is controversial and has not been examined in renal diseases. In this study, we (i) characterised the up-regulation of TGF-s1, phospho-p38 MAPK (p-p38 MAPK) and extracellular matrix during pathogenesis of glomerulonephritis and (ii) examined the effect of rhIL-11 on these processes in vivo. METHODS Following induction of nephrotoxic nephritis, expression of TGF-s1, alpha-smooth muscle actin (alpha-SMA), fibronectin and p-p38 MAPK was detected in the kidney. Rats were treated either with vehicle or rhIL-11 at a high or low dose and culled on day 6. RESULTS A high dose of rhIL-11 resulted in a significant reduction in the glomerular expression of TGF-s1 (0.4 ± 0.1 vs. 2.04 ± 0.4 semiquantitative score, p |
| Databáze: | OpenAIRE |
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