Molecular basis for the resistance of American sloughgrass to aryloxyphenoxypropionic acid pesticides and its environmental relevance: A combined experimental and computational study

Autor: Bing-Qi Liu, Wei Peng, Yu-Kui Peng, Fei Ding, Lingxu Li
Rok vydání: 2019
Předmět:
Models
Molecular
Environmental Engineering
Protein Conformation
Pyridines
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
Environmental pollution
02 engineering and technology
010501 environmental sciences
Biology
Poaceae
01 natural sciences
Gene Expression Regulation
Enzymologic
Gene Expression Regulation
Plant
Environmental Chemistry
Bioassay
Anilides
Pesticides
Plant Proteins
0105 earth and related environmental sciences
Pollutant
Benzoxazoles
Resistance (ecology)
business.industry
Public Health
Environmental and Occupational Health
Computational Biology
General Medicine
General Chemistry
Pesticide
Research findings
Pollution
United States
020801 environmental engineering
Biotechnology
Molecular Docking Simulation
Mutation
Mutagenesis
Site-Directed
Propionates
Target gene
business
Acetyl-CoA Carboxylase
Herbicide Resistance
Zdroj: Chemosphere. 235:1030-1040
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2019.07.044
Popis: Organic pesticides are one of the main environmental pollutants, and how to reduce their environmental risks is an important issue. In this contribution, we disclose the molecular basis for the resistance of American sloughgrass to aryloxyphenoxypropionic acid pesticides using site-directed mutagenesis and molecular modeling and then construct an effective screening model. The results indicated that the target-site mutation (Trp-1999-Leu) in acetyl-coenzyme A carboxylase (ACCase) can affect the effectiveness of the pesticides (clodinafop, fenoxaprop, cyhalofop, and metamifop), and the plant resistance to fenoxaprop, clodinafop, cyhalofop, and metamifop was found to be 564, 19.5, 10, and 0.19 times, respectively. The established computational models (i.e. wild-type/mutant ACCase models) could be used for rational screening and evaluation of the resistance to pesticides. The resistance induced by target gene mutation can markedly reduce the bioreactivity of the ACCase-clodinafop/fenoxaprop adducts, and the magnitudes are 10 and 102, respectively. Such event will seriously aggravate environmental pollution. However, the biological issue has no distinct effect on cyhalofop (RI＝10), and meanwhile it may markedly increase the bioefficacy of metamifop (RI＝0.19). We could selectively adopt the two chemicals so as to decrease the residual pesticides in the environment. Significantly, research findings from the computational screening models were found to be negatively correlated with the resistance level derived from the bioassay testing, suggesting that the screening models can be used to guide the usage of pesticides. Obviously, this story may shed novel insight on the reduction of environmental risks of pesticides and other organic pollutants.
Databáze: OpenAIRE
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