Comparative evaluation of NASBA HIV-1 RNA QT, AMPLICOR-HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma
| Autor: | Georges Zissis, Patrick Lacor, Sabine Lauwers, Hilde Revets, Nathan Clumeck, S De Wit, D. Marissens |
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| Přispěvatelé: | Cellular and Molecular Immunology, Vrije Universiteit Brussel |
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Adult Male Viremia HIV Infections Biology Antiviral Agents Sensitivity and Specificity Virus Virology Blood plasma medicine Humans Sida RNA virus diseases Reproducibility of Results Nucleic acid amplification technique Middle Aged medicine.disease biology.organism_classification NASBA Evaluation Studies as Topic HIV-1 RNA Viral Female Viral disease Nucleic Acid Amplification Techniques Biomarkers Research Article |
| Zdroj: | Vrije Universiteit Brussel |
| ISSN: | 0095-1137 |
| Popis: | Three commercial assays for quantifying plasma human immunodeficiency virus type 1 (HIV-1) RNA were evaluated. The assays differed in their sample volumes, the means of preparing samples, and methods of amplification and detection. Plasma samples were obtained from 36 HIV-1-infected patients representing all stages of HIV-1 infection and were analyzed as coded specimens. Measurement of HIV-1 RNA baseline levels revealed no significant difference in sensitivity between the three assays. The assays were also applied to the quantitation of HIV-1 RNA levels in the plasma of patients who were changing their antiretroviral therapy. The changes measured in HIV-1 RNA levels in plasma in response to therapy were comparable by the three assays. No close correlation was found between the amount of HIV-1 RNA and the CD4 T-cell count; HIV-1 RNA assays were more sensitive than p24 antigen assays as an indicator of plasma viremia. Overall, the study demonstrates that all three quantitative assays for HIV-1 RNA can be used to measure the HIV-1 RNA copy number representing the HIV-1 viremia status in patients with HIV-1 infection. Since this copy number is likely to be useful in monitoring the effectiveness of antiviral therapy, these quantitative assays for HIV-1 RNA are ready to be built into clinical trials. |
| Databáze: | OpenAIRE |
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